RT Journal Article
SR Electronic
T1 Search for airway-derived miRNA biomarkers in pulmonary exacerbation in children with cystic fibrosis
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 3581
DO 10.1183/13993003.congress-2022.3581
VO 60
IS suppl 66
A1 Stachowiak, Z
A1 Narożna, B
A1 Langwiński, W
A1 Wojsyk Banaszak,, I
A1 Jończyk Potoczna, K
A1 Bręborowicz, A
A1 Szczepankiewicz, A
YR 2022
UL https://publications.ersnet.org//content/60/suppl_66/3581.abstract
AB The aim of our study was to verify if extracellular vesicles (EVs)-derived miRNAs are associated with pulmonary exacerbation (PE) in the airways of cystic fibrosis (CF) children. We used 24 sputum and 11 exhaled breath condensate (EBC) CF samples and 4 EBC samples from control subjects. MiRNA expression profile was assessed using next-generation sequencing and differential analysis was done using BaseSpace application “Small RNA” and verified with DESeq2 (Bioconductor). Data were normalized and used for the search of miRNAs with the greatest potential for PE differentiation, as well as the best endogenous control for future studies in sputum (Statistica and Normfinder).Differential analysis in both sputum and EBC did not identify any miRNA that significantly distinguished PE from the stable condition in CF patients. Based on normalized expression and DESeq results, we established a very small number of EBC-derived miRNAs that passed the quality filtering, with the highest expression for hsa-miR-767-3p. The comparison of miRNA expression between EBC from CF and control children did not reveal any significantly altered miRNAs. In sputum samples, hsa-let-7b-5p and hsa-miR-26a-5p were the best endogenous controls.The interindividual differences between CF samples, that result from high diversity and heterogeneity of the disease, limit the identification of miRNAs involved in the airway inflammation in CF children. Further efforts to optimize the isolation of EVs, particularly from EBC samples, will be useful to understand the mechanisms responsible for the course of PE.The study was supported by the Polish National Science Centre, grant no. 2016/22/E/NZ5/00383 (A. Szczepankiewicz).FootnotesCite this article as Eur Respir J 2022; 60: Suppl. 66, 3581.This article was presented at the 2022 ERS International Congress, in session “-”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).