PT  - JOURNAL ARTICLE
AU  - M Scurek
AU  - S Klimovic
AU  - M Pesl
AU  - J Pribyl
AU  - D Kabanov
AU  - D Beckerova
AU  - M Bebarova
AU  - V Rotrekl
AU  - K Brat
TI  - Effects of salbutamol and ipratropium on human stem-cells derived cardiomyocytes’ function
AID  - 10.1183/13993003.congress-2022.560
DP  - 2022 Sep 04
TA  - European Respiratory Journal
PG  - 560
VI  - 60
IP  - suppl 66
4099  - http://erj.ersjournals.com/content/60/suppl_66/560.short
4100  - http://erj.ersjournals.com/content/60/suppl_66/560.full
SO  - Eur Respir J2022 Sep 04; 60
AB  - Introduction: Patients with obstructive pulmonary diseases are treated by beta-2-agonists, parasympatholytics, and methylxanthines for long periods. Chronic use of these drugs may result in impairment of the cardiovascular system. Direct effects of these bronchodilators on the function of human cardiomyocytes are described insufficiently.Aims: We aimed to investigate in vitro effects of salbutamol and ipratropium on human-induced pluripotent stem cell-derived cardiomyocyte (hiPS-CMs) cell clusters. We focused on their chronotropic and inotropic effects.Methods: The measurements of 3D clusters of hiPS-CMs, embryoid bodies (EBs), with spontaneous pacing and contraction abilities were performed using atomic force microscopy (AFM). AFM enables real-time monitoring of EBs’ beating frequency and is capable of measuring its contractility. We measured the effect of increasing concentrations of salbutamol and ipratropium on EBs.Results: We treated 25 EBs with salbutamol and compared results with the control group of 11 EBs. Similarly, we tested 11 EBs with ipratropium. We observed significant positive chronotropic and inotropic effects in higher concentrations of salbutamol with statistically significant elevation of contraction force for clinically relevant salbutamol concentration. Measurements with ipratropium did not reveal any changes in EBs’ electromechanical function.Conclusions: The effect of pulmonary drugs on the cardiovascular system can be reliably tested using a combination of AFM and hiPS-CMs. The clinically relevant concentration of salbutamol causes a significant elevation of human cardiomyocytes’ contractility and beating frequency.Supported by grant MH CZ No NU20-06-00156.FootnotesCite this article as Eur Respir J 2022; 60: Suppl. 66, 560.This article was presented at the 2022 ERS International Congress, in session “-”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).