TY - JOUR T1 - “Deep amplicon sequencing for culture-free prediction of susceptibility or resistance to 13 anti-tuberculous drugs.” A. Jouet, C. Gaudin, N. Badalato, <em>et al</em>. <em>Eur Respir J</em> 2021; 57: 2002338. JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.52338-2020 VL - 60 IS - 5 SP - 2052338 A2 - , Y1 - 2022/11/01 UR - http://erj.ersjournals.com/content/60/5/2052338.abstract N2 - When tracing back source data in response to a reader's request for details of the study, the authors of the abovementioned paper detected a problem with available reference phenotypic data for the comparison with the Deeplex Myc-TB results presented in table 3. This involved mostly (expected) susceptible phenotypes, for which hardly any discordant mutations were found by Deeplex testing, and the problem therefore went unnoticed in the original report.After correction, specificities and sensitivities are only slightly changed compared to previous values for most drugs involved. The overall sensitivity and specificity excluding uncharacterised mutations are 95.2% and 95.0%, respectively (versus 95.3% and 97.4% previously). The slight decrease in overall specificity is mostly a result of the lower specificity (68%) for ethionamide. Importantly, in all discordant cases with resistance predictions by Deeplex Myc-TB versus a susceptible phenotype … ER -