RT Journal Article
SR Electronic
T1 The effects of fluticasone propionate in mice infected with Chlamydia pneumoniae
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA2350
DO 10.1183/13993003.congress-2021.PA2350
VO 58
IS suppl 65
A1 Dóra Paróczai
A1 Anita Sejben
A1 Dávid Kókai
A1 Dezső Virok
A1 Valéria Endrész
A1 Katalin Burián
YR 2021
UL http://erj.ersjournals.com/content/58/suppl_65/PA2350.abstract
AB Introduction: The associations between inhaled corticosteroid (ICS) use and pulmonary infections remains controversial. Chlamydia pneumoniae (C. pneumoniae) accounts for asthma exacerbations, however, there is no data regarding ICS effects on C. pneumoniae infections.Aims: We aimed to investigate whether fluticasone propionate (FP) or budesonide (BUD) could affect C. pneumoniae infection in vivo, focusing on the possible mechanisms that lead to anti-chlamydial outcomes.Methods: FP or BUD were administered by inhalation to C. pneumoniae-infected mice. The mice were sacrificed and their lungs were removed for further investigations. The recoverable C. pneumoniae was determined by indirect immunofluorescence. Expression levels of IFN-γ, and IFN-γ inducible chemokines, indolamine 2,3-dioxygenase (IDO), glucocorticoid receptor (GR) and vitamin D receptor (VDR) were assessed by qPCR. We measured the protein concentrations of IFN-γ and of other cytokines that participate in the anti-chlamydial response by ELISA.Results: FP inhalation reduced C. pneumoniae growth in mice significantly (p<0.001). Higher levels of IFN-γ gene expression were observed in FP-treated mice compared to the untreated and BUD-treated mice (p<0.0001). IFN-γ related IDO expression was also increased in FP treatment (p<0.05). FP enhanced the VDR gene expression (p<0.01). IL-17A and IL-10 production were significantly higher in FP-treated mice than in the untreated group (p<0.05). IFN-γ and MIG/CXCL9 protein levels were significantly higher after FP inhalation, compared to BUD-treated and control mice (p<0.01).Conclusion: FP suppressed C. pneumoniae growth in vivo, due to the enhanced IFN-γ related responses.FootnotesCite this article as: European Respiratory Journal 2021; 58: Suppl. 65, PA2350.This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).