TY - JOUR T1 - T cells subtypes in pleural effusion of tuberculous and non-tuberculous patients JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2021.PA3089 VL - 58 IS - suppl 65 SP - PA3089 AU - Neda Dalil Roofchayee AU - Majid Marjani AU - Neda K.Dezfuli AU - Esmaeil Mortaz Y1 - 2021/09/05 UR - http://erj.ersjournals.com/content/58/suppl_65/PA3089.abstract N2 - Background: Tuberculous pleural effusion (TPE) is one of the most common forms of extrapulmonary tuberculosis. Patients with tuberculous or malignant pleural effusions (MPE) frequently have similar lymphocytic pleural fluid profile.Objective: We aimed to determine whether T helper (Th) subsets able to differentiates TPE from non-TPE patients.Methods: 30 patients with TPE, 30 patients with MPE, 14 patients with EMP and 14 patients with PPE were enrolled between Dec 2018-2019, Tehran-Iran. 3 ml fresh PE harvested and after staining with related Abs, the frequency of T cells phenotypes; CD4+IL-9+, CD4+IL-22+, CD+IL-17+ and CD4+CD25+FOXP3+T cells were determined by flowcytometry.Results: D4+CD25+FOXP3+ T cells were detected as important markers for TPE (P<0.0001). There were a significant differences in of CD4+IL-9+ T cells in TPE in compared to non-TPE (P<0.0001). But, the frequency of CD+IL-17+ and CD4+IL-22+ T cells had no significant difference between TPE and non-TPE patients (P=0.0906 and P=0.2188, respectively).Conclusion: The current data suggests that high frequency of CD4+CD25+FOXP3+ T cells may differentiates TPE from non-TPE and future studies with multicenter studies is needed for generalize this finding as a biomarkers. T cell subtypesTPENon-TPEP valueCD4+IL-9+ (%) 3.67 ( 0.87 – 47.83) 13.05 (1.67 – 61.45)< 0.0001CD4+IL-17+ (%)7.15(1.118– 9.43)15.05 (1.195 -66.55)0.0906CD4+IL-22+ (%)0.2 (0.185 – 61.75)11.8 (1.119 – 70.5)0.2188CD4+CD25+FOXP3+ (%)4.2 (0.362-17.24)26.3 (3.349 – 76.93)<0.0001FootnotesCite this article as: European Respiratory Journal 2021; 58: Suppl. 65, PA3089.This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only). ER -