TY - JOUR T1 - Glycine efficacy for prevention or treatment on LPS-acute lung injury in mice JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2021.PA2351 VL - 58 IS - suppl 65 SP - PA2351 AU - Renato Fraga Righetti AU - Bryan Shinji Haneda Goto AU - Tabata Maruyama Dos Santos AU - Leandro Do Nascimento Camargo AU - Beatriz Mangueira Saraiva-Romanholo AU - Suellen Karoline Moreira Bezerra AU - Edna Aparecida Leick AU - Carla Máximo Prado AU - Milton De Arruda Martins AU - Ruben Siedlarczyk Nogueira AU - José Carlos Sá De Carvalho Neto AU - Daniel Alexandre De Souza AU - Bruno Ramos Salu AU - Maria Luiza Vilela Oliva AU - Iolanda De Fátima Lopes Calvo Tibério Y1 - 2021/09/05 UR - http://erj.ersjournals.com/content/58/suppl_65/PA2351.abstract N2 - Glycine efficacy for prevention or trreatment on LPS-acute lung injury in miceBackground: Glycine showed an important effect on neutrophilic inflammation, involved in ALI/ARDS. Thus it may be a possible therapeutic target of this disease.Objective: to investigate the mechanisms involved in the effect of preventive and therapeutic treatment of glycine in a model of LPS-induced acute lung injury.Methods: Balb/c mice were divided: LPS, LPS-GP, LPS-GT groups received LPS (5mg/kg-intratracheallly). SAL group received saline solution (50μL). LPS-GP: received glycine 1h before LPS administration (1 mg/kg-intraperitoneally). LPS-GT: received glycine 6h after LPS administration (1 mg/kg-intraperitoneally). After 24h, we evaluated: exhaled nitric oxide (eNO), resistance (Rrs) and elastance (Ers) of the respiratory systems and bronchoalveolar lavage fluid (BALF) cells. Statistical significance: ANOVA followed by the Holm-Sidak and considered to be significant when p<0.05.Results: Preventive and therapeutic treatment in LPS-GP (15.0±2.7ppb) and LPS-GT (16.0±2.8ppb) groups decreased the eNO compared to LPS group (28.7±5.1ppb); p<0.05. Considering respiratory mechanics, LPS-GP (1.03±0.01 cmH2O/mL/s) and LPS-GT (0.97±0.04 cmH2O/mL/s) groups decreased the Rrs compared to LPS group (1.4±0.1 cmH2O/mL/s) and LPS-GP (50.1±3.7 cmH2O/mL) and LPS-GT (50.6±4.4 cmH2O/mL) groups decreased the Ers compared to LPS group (93.2±7.1 cmH2O/mL); p<0.05. LPS-GP (10.4±2.3 x104cells/mL) and LPS-GT (8.8±1.6 x104cells/mL) groups decreased the total cells in BALF compared to LPS group (24.8±7.0 x104cells/mL); p<0.05.Conclusion: Preventive and therapeutic treatment with glycine plays an important role in protecting the ALI induced by LPS.FootnotesCite this article as: European Respiratory Journal 2021; 58: Suppl. 65, PA2351.This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only). ER -