TY - JOUR T1 - Late Breaking Abstract - A multiomic micro- and macro-anatomical cell atlas of the human lungs and airways JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2021.OA1374 VL - 58 IS - suppl 65 SP - OA1374 AU - Kerstin Meyer AU - Elo Madisoon AU - Anna Wilbrey-Clark AU - Vitalii Kleshchevnikov AU - Ana Ribeiro Orsi AU - Krzysztof Polanski AU - Lira Mamanova AU - Liam Bolt AU - Amanda Oliver AU - Natsuhiko Kumasaka AU - Ni Huang AU - Rasa Elmentaite AU - Juliana Weller AU - Jan Patrick Pett AU - Peng He AU - Monika Dabrowska AU - Liz Tuck AU - Elena Prigmore AU - Andrew Knights AU - Sophie Pritchard AU - Adam Hunter AU - Sarah Vieira AU - Minal Patel AU - Nikitas Georgakopoulos AU - Krishnaa Muhbubani AU - Kourosh Saeb-Parsy AU - Oliver Stegle AU - Sarah Teichmann Y1 - 2021/09/05 UR - http://erj.ersjournals.com/content/58/suppl_65/OA1374.abstract N2 - To better understand the cell types of the lung and how they relate to one another, we have generated a multi-omics data set from 5 different locations of the lung coupled with Visium 10X spatial transcriptomics analysis. Our analysis has generated 190,000 high quality single cell and nuclear transcriptomes with even contribution from trachea, bronchi, bronchioli, upper and lower parenchyma.Nuclear sequencing allowed us to identify otherwise hard to dissociate cell types and we report transcriptomes for lung chondrocytes that has been missing from earlier data sets. Within the epithelial compartment, we define submucosal gland (SMG) cell populations, comprising mucous, serous, duct as well as myoepithelial cells. Using Visium 10X we map all the SMG cell types to separate locations, providing orthogonal evidence that these clusters represent distinct cell types. We report changes in cell type composition with location and found that ciliated cells in the trachea expressed higher levels of ACE2, the receptor for SARS-CoV-2. Similarly, we define novel fibroblast populations, such as the peribronchial, perichondrial and neural associated fibroblasts, as well as myelinating and non-myelinating Schwann cells, which express peripheral neuropathy associated genes. We have generated a detailed annotation of lung-resident immune cells, with increased granularity particularly in the macrophage compartment, and have initiated an analysis of the molecular pathways that determine the location of immune cells to sub-locations within the lungs and airways.Our work has defined novel cell types in the lung and airways and mapped these into the tissue context.FootnotesCite this article as: European Respiratory Journal 2021; 58: Suppl. 65, OA1374.This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only). ER -