RT Journal Article
SR Electronic
T1 Characterization of the resistance mechanisms to carbapenems and virulence genes in Pseudomonas aeruginosa strains from children with cystic fibrosis
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA3367
DO 10.1183/13993003.congress-2021.PA3367
VO 58
IS suppl 65
A1 Zulfirya Sadeeva
A1 Natalya Alyabyeva
A1 Irina Novikova
A1 Anna Lazareva
A1 Yliya Gorinova
A1 Olga Simonova
YR 2021
UL http://erj.ersjournals.com/content/58/suppl_65/PA3367.abstract
AB Introduction: P. aeruginosa(PA) infection poses a serious risk to patients with cystic fibrosis (CF). The course and antibacterial therapy of such infections depends not only on the resistance, but also on the virulent properties of the strains.Objectives: To determine the susceptibility and resistance mechanisms to carbapenems, virulence genes exoU, exoS.Methods: For the period 2017-2019 167 strains of PA were studied. Antibiotic sensitivity was determined by broth microdilution. Carbapenemases were detected by real-time polymerase chain reaction (PCR). Virulence genes were determined by PCR followed by electrophoresis detection in a 2% agarose gel.Results: Of the studied PA isolates, 69 (41%) were classified as insensitive to carbapenems. The blaVIM gene was found in ten isolates (6%). Other metallo-beta-lactamases (MBL) were not detected. The exoU virulence gene was identified in 22 (13%) PA strains, of which 12 (7%) isolates were insensitive to carbapenems. The exoS virulence gene was detected in 56 (34%) strains, among them 16 (9%) isolates were resistant to carbapenems. A combination of exoU and exoS genes was observed in one sensitive and one resistant strains. No virulence genes were found in 90 (54%) isolates.Conclusions: The resistance of PA isolates isolated from children with CF is rarely associated with the presence of MBL (6%). The presence of virulence genes does not correlate with the resistance of the strains. The exoS gene is more common in carbapenem-susceptible strains than in carbapenem-resistant strains. The exoU gene is found with equal frequency in strains sensitive and insensitive to carbapenems.FootnotesCite this article as: European Respiratory Journal 2021; 58: Suppl. 65, PA3367.This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).