RT Journal Article
SR Electronic
T1 Inflammation regulating cytokines in TB affected tissues
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA2466
DO 10.1183/13993003.congress-2021.PA2466
VO 58
IS suppl 65
A1 Galina Shepelkova
A1 Vladimir Evstifeev
A1 Ruslan Tarasov
A1 Mamed Bagirov
A1 Vladimir Yeremeev
YR 2021
UL http://erj.ersjournals.com/content/58/suppl_65/PA2466.abstract
AB Introduction: Cytokines play important role in regulating infection without promoting uncontrolled and damaging inflammatory responses. Herein we focus on the key cytokines and chemokines expressed at the site of infection – in the lungs of TB patients.Aims and objectives: We compared the levels of cytokine/chemokine gene expression in the tuberculoma/caverna wall and in relatively intact tissue at the periphery of resected specimen from post-primary TB patients.Methods: Written informed consent was obtained from each participant prior to biopsy collection. The expression levels of cytokines/chemokines genes were analyzed by TaqMan PCR Assay. ELISA was used to estimate respective proteins in tissue homogenates.Results: Most prominent differences in cytokine/chemokine genes expression were registered when FCT cavity wall and parenchyma tissues were compared. Cxcl1 (p ≤ 0.03) and Cxcl2 (p ≤ 0.01), IL6 (p≤0.0001), IL8 (p ≤0.01) were upregulated in parenchymatous tissue as compared to cavity wall. Destruction processes in tuberculoma patients correlate with the levels of cell migration factors such as IL-6, G-CSF and MIP-1a.Conclusions: Our data indicate significant changes in the levels of certain cytokines/chemokines in the lungs of patients with post-primary TB. These changes primarily concern cytokines/chemokines involved in the regulation of specific inflammation, cell migration and fibrosis formation.This work was supported by the Scientific Program SR 0515-2019-0018.FootnotesCite this article as: European Respiratory Journal 2021; 58: Suppl. 65, PA2466.This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).