PT  - JOURNAL ARTICLE
AU  - Sara Zanella
AU  - Volker Strenger
AU  - Walter Buzina
AU  - Sophie Kienreich
AU  - Markus Egger
AU  - Andreas Pfleger
AU  - Ernst Eber
TI  - Late Breaking Abstract - Can individualised skin prick tests detect sensitisations against colonising fungal species in cystic fibrosis patients?
AID  - 10.1183/13993003.congress-2021.PA3381
DP  - 2021 Sep 05
TA  - European Respiratory Journal
PG  - PA3381
VI  - 58
IP  - suppl 65
4099  - http://erj.ersjournals.com/content/58/suppl_65/PA3381.short
4100  - http://erj.ersjournals.com/content/58/suppl_65/PA3381.full
SO  - Eur Respir J2021 Sep 05; 58
AB  - Introduction: The lack of skin prick test (SPT) extracts or specific IgE tests against most fungi possibly leads to underestimating allergic bronchopulmonary mycosis (ABPM) in cystic fibrosis (CF) patients.Objective: We aimed to investigate if CF patients are sensitised against fungal species colonising their airways and if a sensitisation is detectable via SPTs using individually produced extracts.Methods: In this prospective study, individually produced extracts from colonising fungi, and Aspergillus fumigatus and Candida albicans commercial extracts were used to perform SPTs.Results: 44 out of 111 patients were colonised with relevant amounts of fungi and tested with individually produced extracts of 20 colonising fungal species. The SPTs of 16 out of 44 (36.4%) patients resulted positive: 3 were positive to individually produced and commercial extracts of A. fumigatus. 1 was positive to individually produced and commercial extracts of C. albicans, and to an individually produced extract of A. fumigatus “non-sporulating” subtype, but negative to A. fumigatus “sporulating” subtype and commercial extracts. 12 patients were positive to commercial extracts (10 to A. fumigatus, 2 to C. albicans) but were not colonised and therefore not tested with individually produced extracts. Only in 1 out of 28 cases (3.6%) a discordant result between commercial and individually produced extracts was observed.Conclusion: No sensitisation to other fungi than A. fumigatus or C. albicans was found. This may be due to other fungi not inducing sensitisation or due to the low number of colonised patients. The method appears to be reliable but further studies are needed.FootnotesCite this article as: European Respiratory Journal 2021; 58: Suppl. 65, PA3381.This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).