RT Journal Article
SR Electronic
T1 Periostin: contributor to abnormal airway epithelial function in asthma?
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 2001286
DO 10.1183/13993003.01286-2020
VO 57
IS 2
A1 Janette K. Burgess
A1 Marnix R. Jonker
A1 Marijn Berg
A1 Nick T.H. ten Hacken
A1 Kerstin B. Meyer
A1 Maarten van den Berge
A1 Martijn C. Nawijn
A1 Irene H. Heijink
YR 2021
UL http://erj.ersjournals.com/content/57/2/2001286.abstract
AB Periostin (POSTN) may serve as a biomarker for Type-2 mediated eosinophilic airway inflammation in asthma. We hypothesised that a Type-2 cytokine, interleukin (IL)-13, induces airway epithelial expression of POSTN, which in turn contributes to epithelial changes observed in asthma.We studied the effect of IL-13 on POSTN expression in BEAS-2B and air–liquid interface differentiated primary bronchial epithelial cells (PBECs). Additionally, the effects of recombinant human POSTN on epithelial-to-mesenchymal transition (EMT) markers and mucin genes were assessed. POSTN single cell gene expression and protein levels were analysed in bronchial biopsies and induced sputum from asthma patients and healthy controls.IL-13 increased POSTN expression in both cell types and this was accompanied by EMT-related features in BEAS-2B. In air–liquid interface differentiated PBECs, IL-13 increased POSTN basolateral and apical release. Apical administration of POSTN increased the expression of MMP-9, MUC5B and MUC5AC. In bronchial biopsies, POSTN expression was mainly confined to basal epithelial cells, ionocytes, endothelial cells and fibroblasts, showing higher expression in basal epithelial cells from asthma patients versus those from controls. A higher level of POSTN protein expression in epithelial and subepithelial layers was confirmed in bronchial biopsies from asthma patients when compared to healthy controls. Although sputum POSTN levels were not higher in asthma, levels correlated with eosinophil numbers and with the coughing-up of mucus.POSTN expression is increased by IL-13 in bronchial epithelial cells and is higher in bronchial biopsies from asthma patients. This may have important consequences, as administration of POSTN increases epithelial expression of mucin genes, supporting the relationship of POSTN with Type-2 mediated asthma and mucus secretion.High levels of IL-13 in Type-2 driven asthma play a crucial role in the development of the abnormal epithelial phenotype by induction of periostin (POSTN) secretion. POSTN induces mucin production in airway epithelial cells. https://bit.ly/3hg7ETu