RT Journal Article
SR Electronic
T1 Cross-reactive antibodies against dust mite-derived enolase induce neutrophilic airway inflammation
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1902375
DO 10.1183/13993003.02375-2019
VO 57
IS 1
A1 Lin, Jianli
A1 Huang, Nana
A1 Li, Jing
A1 Liu, Xiaoyu
A1 Xiong, Qing
A1 Hu, Chengshen
A1 Chen, Desheng
A1 Guan, Lvxin
A1 Chang, Kexin
A1 Li, Dan
A1 Tsui, Stephen Kwok-Wing
A1 Zhong, Nanshan
A1 Liu, Zhigang
A1 Yang, Ping-Chang
YR 2021
UL http://erj.ersjournals.com/content/57/1/1902375.abstract
AB Background and aims Neutrophilic inflammation is a hallmark of some specific asthma phenotypes; its aetiology is not yet fully understood. House dust mite (HDM) is the most common factor in the pathogenesis of airway inflammation. This study aims to elucidate the role of cross-antibodies against HDM-derived factors in the development of neutrophilic inflammation in the airway.Methods Blood samples were collected from asthma patients with chronic neutrophilic asthma for analysis of HDM-specific cross-reactive antibodies. The role of an antibody against HDM-derived enolase (EnoAb) in the impairment of airway epithelial barrier function and induction of airway inflammation was assessed in a cell culture model and an animal model.Results High similarity (72%) of the enolase gene sequences was identified between HDM and human. Serum EnoAb was detected in patients with chronic neutrophilic asthma. The EnoAb bound to airway epithelial cells to form complexes with enolase, which activated complement, impaired airway epithelial barrier functions and induced neutrophilic inflammation in the airway tissues.Conclusions HDM-derived enolase can induce specific cross-antibodies in humans, which induce neutrophilic inflammation in the airway.House dust mite (HDM)-derived enolase induces cross-antibodies in the body, which recognise both HDM-derived enolase and the enolase in the airway epithelial cells, activate complement and induce neutrophilic inflammation in the airway tissues https://bit.ly/2XKSCgy