PT  - JOURNAL ARTICLE
AU  - Grigory Kaminskiy
AU  - Anna Panova
AU  - Eduard Karamov
AU  - Elena Veselova
AU  - Sergey Smerdin
AU  - Marina Leichliyader
AU  - Anastasia Samoilova
AU  - Irina Vasilyeva
TI  - Induction of M. tuberculosis (MTB) drug resistance (DR) mutations in HIV-infected hosts and pathways of further spread of the evolutionary successful drug-resistant MTB clonal lines
AID  - 10.1183/13993003.congress-2020.1596
DP  - 2020 Sep 07
TA  - European Respiratory Journal
PG  - 1596
VI  - 56
IP  - suppl 64
4099  - http://erj.ersjournals.com/content/56/suppl_64/1596.short
4100  - http://erj.ersjournals.com/content/56/suppl_64/1596.full
SO  - Eur Respir J2020 Sep 07; 56
AB  - Introduction: DR and HIV are the major challenges for worldwide eradication of tuberculosis (TB).Aims: To determine MTB DR mutations, prevalent in HIV+ and HIV- individuals; to identify MTB clonal lines evolutionary successful with respect to DR mutations.Methods: MTB cultures were isolated from symptomatic patients (93 HIV+ and 267 HIV-) before the onset of TB treatment and subjected to biochip hybridisation.Results: Latter DR mutations were statistically significant prevalent in HIV+ as compared to HIV- individuals: - S531L rpoB (rifampicin resistance),- M306V embB (etambutol resistance),- ‘S531L rpoB + S315T(1) katG’ (isoniazid resistance),- ‘S531L rpoB + S315T(1) katG + M306V embB’.The prevalence in HIV- individuals of S315T(1) katG not combined with S531L rpoB was statistically significant.The prevalence in HIV+ patients of the MDR marker ‘S531L rpoB + S315T(1) katG’ was demonstrated for all genotypes; its proportion amounted to 100% in B0/W148 genotype.Two distinct evolutionary successful MDR clonal lines originating in Chelyabinsk were discovered: 1)  Beijing genotype with the marker ‘S531L rpoB + S315T(1) katG + M306V embB’; spread to Kemerovo, Leningrad, Stavropol, and Nizhnii Novgorod regions; 8 out of 19 (42%) cultures were XDR;2)  B0/W148 genotype with the marker ‘S531L rpoB + S315T(1) katG + N296H embB’, spread to Kemerovo, Leningrad, Nizhnii Novgorod, and Moscow regions; 2 out of 12 (17%) cultures were XDR.Conclusion: MTB passage through HIV+ patients induces new MDR (and potentially XDR) clonal lines, which retain tropism to HIV+ patients even after passage through HIV- individuals.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 1596.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).