RT Journal Article
SR Electronic
T1 Upregulation of Suppressors of Cytokine Signalling (SOCS)3 in COPD alveolar macrophages
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 2284
DO 10.1183/13993003.congress-2020.2284
VO 56
IS suppl 64
A1 Bazzan, Erica
A1 Tinè, Mariaenrica
A1 Turato, Graziella
A1 Semenzato, Umberto
A1 Romagnoli, Micaela
A1 Cosio, Manuel
A1 Saetta, Marina
YR 2020
UL http://erj.ersjournals.com/content/56/suppl_64/2284.abstract
AB Background: The Suppressor of Cytokine Signalling (SOCS) proteins, particularly SOCS3, and its regulation by TNF-α, have been originally described in animal and in vitro studies as inhibitors of cytokine-signalling in response to a chronic inflammation. However, more recently, the suppressive role of SOCS3 has been questioned by other animal studies, which suggested an important proinflammatory role for this protein.Aim: To investigate in a human inflammatory disease, COPD, the expression of SOCS3 and its inducer TNF-α in alveolar macrophages (AM).Methods: SOCS3 and TNF-α expression was quantified in alveolar macrophages (SOCS3+AM%, TNF-α+AM%) in lungs from 19 smokers with COPD (COPD) [FEV1: 60±31(%)], 16 smokers without COPD (noCOPD) and 9 nonsmokers (NS) using immunochemistry.Results: The percentage of SOCS3+AM was higher in COPD [68(31-95)%] than in noCOPD [48(22-78)% p=0.04] and NS [10(4-56)% p=0.005]. This pattern was paralleled by the expression of TNF-α in AM, that was higher in COPD [90(52-97)%] than in noCOPD [32(17-60)%] and in NS [1(0-15)% p<0.001]. In alveolar macrophages, TNF-α expression was correlated with that of SOCS3+ (r=0.5; p=0.02).Conclusions: The increased expression of SOCS3 and its modulator TNF-α in alveolar macrophages of COPD, a chronic inflammatory disease, casts some doubts about SOCS3 suppressor function and rather favours a role for SOCS3 as a proinflammatory mediator.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 2284.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).