RT Journal Article
SR Electronic
T1 Exhaled breath particles - a potential noninvasive method to detect and monitor chronic allograft dysfunction in lung transplant recipients
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 4727
DO 10.1183/13993003.congress-2020.4727
VO 56
IS suppl 64
A1 Anna Niroomand
A1 Oskar Hallgren
A1 Darcy Wagner
A1 Sandra Lindstedt
YR 2020
UL http://erj.ersjournals.com/content/56/suppl_64/4727.abstract
AB Chronic lung allograft dysfunction (CLAD) is a severely limiting factor to the long-term survival of lung transplant recipients (LTx). Detection of CLAD is hampered by a lack of clinically available markers. Exhaled breath particles (EBP) is proposed as a noninvasive means of identifying CLAD patients and observing their condition. This pilot study aims to capture the range of EBP expected in human samples and identify possible candidate markers for further study.EBP are collected using a PExA device. Samples were obtained from healthy subjects (a combined sample of 600 ng and a 200 ng from each) and from five patients (one grouped sample of 600 ng from three patients and two 200 ng samples from two unique patients). The samples were analyzed via tandem mass spectrometry (MS).Up to 650 proteins were identified in the EBP samples identified. The fifty proteins identified represented a breadth of biological function and displayed a variety of localizations. Ten could be traced back to immunological system processes and included a number of immunoglobulin constant regions. Sixteen of these proteins could be found in the extracellular region.EBP presents as a novel method for the non-invasive analysis of LTx with the potential to monitor for the development of CLAD. This pilot study characterizes the range of proteins that can be identified from samples across subjects and serves as the basis for the exploration of markers in LTx. The findings of this work serve as the establishing method for the ongoing effort to use EBP to identify and monitor CLAD in LTx recipients.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 4727.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).