PT  - JOURNAL ARTICLE
AU  - Fedorchenko, Yuliya
AU  - Zaiats, Lubomyr
TI  - State of the cellular components of the surfactant system in case of experimental diabetes mellitus
AID  - 10.1183/13993003.congress-2020.2571
DP  - 2020 Sep 07
TA  - European Respiratory Journal
PG  - 2571
VI  - 56
IP  - suppl 64
4099  - http://erj.ersjournals.com/content/56/suppl_64/2571.short
4100  - http://erj.ersjournals.com/content/56/suppl_64/2571.full
SO  - Eur Respir J2020 Sep 07; 56
AB  - Objective: Diabetes mellitus nowadays remains an important medical and social problem. To study the ultrastructural changes of alveolocytes of the II type (A II) in case of experimental diabetes mellitus in dynamics.Methods: Experiments were carried out on 30 white male Wistar rats, 170-210 grams weight. Diabetes mellitus was induced by intraperitoneal injection of streptozotocin solution (`Sigma`, USA), diluted in 0.1 M citrate buffer with pH 4,5 in dose of 60 mg/kg of weight. Pulmonary tissue sample acquirement for electronic microscopic research was performed under thiopental narcosis after 42, 56 and 70 days. Pulmonary tissue samples were fixed in 2,5% glutaraldehyde with further co-fixation in 1% osmium tetroxide. After dehydration the material was put into epon-araldite. Cuts, acquired on the ultramicrotome `Tesla BS-490`, were studied on the electronic microscope `PEM-125K`.Results: Conducted electron microscope analysis showed, that the most expressed structural changes were observed in the distant terms of the diabetes mellitus (56-70 days of diabetes). Swollen mitochondria with solitary cristae were revealed in the cytoplasm of alveolocytes of the II type (A II). Cisterns of Goldzhi apparatus alongside with rough endoplasmic reticulum were fragmentated in most cases. Lamellar bodies were vacuolized with fragments of osmophilic plates inside. Apical surface of alveolocytes of the II type (A II) is smoothed by depletion of the numbers of microvilli.Conclusions: Streptozotocin-induced diabetes mellitus is accompanied by expressed ultrastructural changes of alveolocytes of the II type , which supports disorders in surfactant system of lungs.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 2571.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).