PT - JOURNAL ARTICLE AU - Chun Sang Pun AU - Hai Chao Wang AU - Kin Pong Tao AU - Gar Shun Tsun AU - Wai Yin Yu AU - Xia Yuan Chen AU - Maggie Haitian Wang AU - Paul Kay-Sheung Chan AU - Ting Fan Leung AU - Wan Yi Renee Chan TI - Discovering the molecular determinant in rhinoviruses by virus-genotype to disease-phenotype association in paediatrics patients in Hong Kong AID - 10.1183/13993003.congress-2020.1152 DP - 2020 Sep 07 TA - European Respiratory Journal PG - 1152 VI - 56 IP - suppl 64 4099 - http://erj.ersjournals.com/content/56/suppl_64/1152.short 4100 - http://erj.ersjournals.com/content/56/suppl_64/1152.full SO - Eur Respir J2020 Sep 07; 56 AB - Rhinovirus (RV) infection is one of the causes of common cold. A more severe disease spectrum has found to be associated with paediatrics patients after the discovery of new RV species C in 2010, particularly on the development of asthma in early life exposure.It is hypothesised that the 168 rhinovirus-genotypes may induce different biological pathways, resulting in distinct disease phenotypes. This study aims to identify the molecular determinants in the RV-genotypes to understand the virus-genotype to disease-phenotype association.1,133 nasopharyngeal aspirate (NPA) frozen archive specimens were collected from hospitalised children aged 1 to 17 in two regional tertiary hospitals in Hong Kong from 2015 to 2016. The specimens were found to be enterovirus positive by multiplex-PCR. Sanger sequencing was done on the VP4/VP2 viral protein region, and the RV genotype was identified by BLAST+. The symptoms of diagnosis in the discharge notes were reviewed and categorised into upper respiratory tract infections (URTI), pneumonia, asthma exacerbation, and acute bronchiolitis. The association of virus-genotype to diseases-phenotype were calculated by Fisher’s exact test.Among 1,133 cases, 870 cases passed our in-house database. 16 genotypes were found to be associated with URTI, pneumonia, asthma exacerbation, and acute bronchiolitis, respectively.The highly associated genotype will undergo complete viral genome sequencing. By aligning with the viral strains, unique molecular determinants that are related to the diseases-phenotype may be identified. The biological implication of the determinants will be useful for curing diseaes and drug discovery.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 1152.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).