%0 Journal Article %A Silvia Sánchez-Díez %A María Jesús Cruz %A Daniel Álvarez-Simon %A Tomás Montalvo %A Xavier Muñoz %A Susana Gómez-Ollés %T A rapid test for environmental detection of pigeon antigen %D 2020 %R 10.1183/13993003.congress-2020.1994 %J European Respiratory Journal %P 1994 %V 56 %N suppl 64 %X Introduction: Bird-related hypersensitivity pneumonitis is an interstitial lung disease induced by avian proteins, specially pigeon antigens. Complete avoidance to inhaled antigens is an essential step to ameliorate respiratory symptoms and prevent disease progression. The aim of the present study was to develop a lateral flow immunoassay (LFIA) method to detect pigeon antigens in environmental air samples.Methods: Twenty-two different air samples were collected from two different dovecotes of the province of Barcelona with an automated air sampler. Pigeon allergens were mixed with labeled specific anti-pigeon antibodies. The immunocomplexes formed migrate through a nitrocellulose membrane and bound to a line of immobilized specific anti-pigeon antibodies giving a visible band. Strip density values were calculated and compared with the concentration determined by an already standardized ELISA method for pigeon antigens. LFIA strips results were also visually analysed by five independent evaluators.Results: LFIA assay was able to detect a range of concentrations from 420 to 3360 ng/ml of pigeon antigen. A strong correlation was observed between the concentration determined by the ELISA method and the relative density results of the LFIA (rs:0.935; p<0.0001). Bland-Altman plot also confirmed a good agreement between both methods (mean difference: -1.626; p<0.0001). A kappa index of 0.736 (p<0.0001) was obtained between the observers evaluating the strip results.Conclusions: A rapid and easy semiquantitative pigeon allergen estimation method was developed. There was a good correlation between results obtained with ELISA method and LFIA assay.Study funded by ISCIII (PI15 / 01954), FEDER and FUCAP.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 1994.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only). %U