PT  - JOURNAL ARTICLE
AU  - de Souza Xavier Costa, Natália
AU  - Da Costa Sigrist, Giovana
AU  - Dolhnikoff, Marisa
AU  - Ferraz Da Silva, Luiz Fernando
TI  - RAGE expression in lung tissue of ARDS and septic patients
AID  - 10.1183/13993003.congress-2020.2752
DP  - 2020 Sep 07
TA  - European Respiratory Journal
PG  - 2752
VI  - 56
IP  - suppl 64
4099  - http://erj.ersjournals.com/content/56/suppl_64/2752.short
4100  - http://erj.ersjournals.com/content/56/suppl_64/2752.full
SO  - Eur Respir J2020 Sep 07; 56
AB  - Background: Several studies have indicated the involvement of receptor for advanced glycation end products (RAGE) in the pathophysiology of ARDS and its potential as biomarker, however there are scarce information on the expression profile of RAGE in lung tissue of ARDS patients.Aims: To assess the alveolar epithelium expression of membrane-bound RAGE of patients with ARDS and sepsis.Methods: Using immunohistochemistry and image analysis, we quantified membrane-bound RAGE expression in the lung epithelium of patients who died of ARDS (with or without sepsis; classified according to the Berlin definition; n=13) and sepsis (without criteria for ARDS; n=15) who had their autopsies performed at the São Paulo Autopsy Service (Brazil) and correlated it with clinical variables.Results: The severe ARDS patients (0.36ǂ0.02) had higher RAGE expression than the mild ARDS (0.19±0.02; p=0.001) and the septic patients (0.25±0.04; p=0.019). The septic patients had the expression of RAGE negatively correlated with hospitalization days (r=-0.667; p=0.007) and positively correlated with serum lactate levels (r=0.658; p=0.02) and hematocrit (r=0.75; p=0.012). In ARDS patients, the alveolar epithelium RAGE expression is negatively correlated with hospitalization days (r=-0.685; p=0.010) and with the PaO2/FiO2 ratio (r=-0.656; p=0.021).Conclusion: Our results show that the membrane-bound RAGE epithelium expression is higher in patients with severe ARDS than in septic patients, and it correlates with ARDS severity. These results corroborate previous studies that show that patients with severe ARDS have increased levels of soluble RAGE, reinforcing the evidence that it could be a valuable biomarker candidate.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 2752.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).