TY - JOUR T1 - Whole genome sequencing of the highly virulent subtype B0/W148 of the Beijing genotype M.tuberculosis in Kharkiv, East Ukraine JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2020.1448 VL - 56 IS - suppl 64 SP - 1448 AU - Olha Konstantynovska AU - Petro Poteiko AU - Oleksandr Zinenko AU - Anton Rohozhyn AU - Ivan Hrek AU - Nataliia Rudova AU - Anton Gerilovych AU - Gerald Fischer AU - Luke T. Daum AU - Oleksii Solodiankin Y1 - 2020/09/07 UR - http://erj.ersjournals.com/content/56/suppl_64/1448.abstract N2 - The number of Beijing-like M.tuberculosis (MTB) genotype strains among pulmonary tuberculosis (TB) patients in Kharkiv, Ukraine has doubled from 32.1% to 62% in the last 10 years. Previously, we reported the emergence of genotype 42425 in Kharkiv region, which was detected using the MIRU-VNTR analysis (A, B, C, D, E loci). These findings coincided in profile (15 loci) with the highly virulent MTB subtype of the Beijing B0/W148 genotype. To confirm our findings, we performed whole genome sequencing (WGS) of 42425 genotype.Materials and Methods: In 2018, a total of 100 clinical isolates obtained from pulmonary TB patients were collected in Kharkiv, Ukraine and transferred to cryotubes containing PrimeStore Molecular Transport Medium® (Longhorn Vaccines & Diagnostics, San Antonio, TX). The isolates were transported from Kharkiv, Ukraine, to San Antonio, TX at ambient temperature. WGS was performed using an Illumina MiSeq platform. Bioinformatics analysis was conducted using the PhyResSE and Geneious Prime.Results: For genomic analysis, 8 isolates with a maximum reading depth were selected and also genotyped by MIRU-VNTR analysis as the Beijing group strains. Kharkiv isolates are similar to the B0/W148 subtype and contained two large inversions in the genome. As in subtype B0/W148, blocks 1, 3 and 5 maintained their positions. According to point mutation analysis, the Kharkiv isolates were resistant to all first line drugs, fluoroquinolones and aminoglycosides.Conclusions: Circulation of highly virulent of MTB strains may be a contributing factor in the XDR-TB outbreak in East Ukraine and should be further studied to improve TB diagnostics for better outcomes.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 1448.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only). ER -