RT Journal Article
SR Electronic
T1 Novel swine ARDS model induced by Pseudomonas aeruginosa pneumonia and VILI
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 2755
DO 10.1183/13993003.congress-2020.2755
VO 56
IS suppl 64
A1 Ana Motos Galera
A1 Enric Barbeta
A1 Joaquim Bobi
A1 Hua Yang
A1 Minlan Yang
A1 Giacomo Tanzella
A1 Roberto Cabrera
A1 Carmen Rosa Vargas
A1 Pierluigi Di Ginnatale
A1 Stefano Nogas
A1 Marta Arrieta
A1 Laia Fernandez-Barat
A1 Ricard Mellado
A1 Montserrat Rigol
A1 Gerard Frigola
A1 Miquel Ferrer
A1 Gianluigi Li Bassi
A1 Antonio Artigas
A1 Antoni Torres
YR 2020
UL http://erj.ersjournals.com/content/56/suppl_64/2755.abstract
AB Background: Translational animal models of acute respiratory distress syndrome (ARDS) do not completely reproduce all features of human ARDS1. These models are usually rapidly reversible and time-limited1.Aims: To develop a novel swine ARDS model that mimics pathological features.Methods: Mechanically ventilated pigs were challenged with a 107CFU/mL P. aeruginosa culture after 3 hours of ventilation-induced lung injury (VILI). Following the inoculum, animals underwent again to VILI until diagnosis criteria were achieved (PaO2/FiO2<150). Dual antimicrobial therapy was started when pneumonia was clinically confirmed. Hemodynamic parameters, respiratory mechanics, and gas exchange were evaluated throughout the study. Ventilator settings were adjusted and clinical sepsis guidelines applied to reach respiratory and hemodynamic stability. Sixty hours after diagnosis lungs were harvested for histology.Results: Four animals (45.3±2.4 kg) completed the study, while one animal was euthanized 30h after ARDS diagnosis due to severe respiratory instability. ARDS diagnosis criteria were achieved after 32.7±4.4 h of VILI. Figure 1 displays the main outcomes. Of note, diffuse alveolar damage was found on tissue samples.Conclusions: This long-lasting swine ARDS model induced by P. aeruginosa pneumonia and VILI reproduces the clinical features found in ARDS patients. Inflammatory response is still under investigation.110.1016/j.athoracsur.2011.06.107FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 2755.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).