RT Journal Article
SR Electronic
T1 Quantitative LC-MS/MS identifies significantly altered protein profiles in bronchial lavage fluid in non-allergic and allergic asthma
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 4082
DO 10.1183/13993003.congress-2020.4082
VO 56
IS suppl 64
A1 Emma Boberg
A1 Cecilia Lässer
A1 Carina Malmhäll
A1 Kristina Johansson
A1 Henric Olsson
A1 Madeleine Rådinger
YR 2020
UL http://erj.ersjournals.com/content/56/suppl_64/4082.abstract
AB Background: There is an unmet need to understand the underlying mechanisms contributing to the pathogenesis in asthma subgroups. Thus, in the current study we aimed to determine protein profiles in bronchial lavage (BL) fluid obtained from non-allergic (NAA) and allergic (AA) asthma groups and healthy controls.Methods: BL was collected from 20 well-characterized subjects (6-7/group) from the cohort West Sweden Asthma Study (WSAS). Proteins were quantified by LC-MS/MS. The protein expression profiles were examined using the software Qlucore Omics Explorer.Results: In total 3467 proteins were quantified and 2962 were quantified in all samples. The majority of the significantly altered proteins (p<0.01) were downregulated in the two asthma groups compared to healthy controls. However, comparing NAA subjects with AA subjects, distinct unique proteins were identified distinguishing the two asthma groups. Fifty proteins were significantly downregulated in NAA compared to AA. These proteins were associated with GO term connected to ciliogenesis, mRNA splicing and lung epithelial cell differentiation. Eight proteins were significantly upregulated in NAA compared to AA and two of these were connected to the classical complement pathway.Conclusion: The two asthma groups display a unique proteomic profile compared to healthy controls. Interestingly, distinct protein profiles were identified, separating the two asthma groups. These findings need to be validated and further explored in order to identify new disease mechanisms that distinguish different asthma groups.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 4082.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).