RT Journal Article
SR Electronic
T1 Activation of toxicology-related genes in human airway constructs after exposure to indoor air particulate matter from moisture-damaged houses
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 4923
DO 10.1183/13993003.congress-2020.4923
VO 56
IS suppl 64
A1 Maria-Elisa Nordberg
A1 Martin Täubel
A1 Hanna Leppänen
A1 Anne Hyvärinen
A1 Kati Huttunen
YR 2020
UL http://erj.ersjournals.com/content/56/suppl_64/4923.abstract
AB Exposure to altered microbial characteristics as a consequence of moisture damage indoors is considered to be a key factor behind the observed health effects. Nevertheless, the connection between moisture damage, microbial exposure, adverse health effects and pathophysiological mechanisms is still unknown. Our aim was to study toxicology-related gene expression in airway epithelium after exposure to indoor particulate matter (PM) sampled from moisture-damaged houses.Normal human bronchial epithelial cells (NHBE) were differentiated in air-liquid interface for 22 days. The airway constructs were exposed (24 h) to three doses of indoor PM (1:16, 1:8, 1:4) sampled from nine moisture-damaged vs. non-moisture-damaged -house pairs, each pair having similar construction, age, and geographical location. The RNA of the airway constructs was extracted and sequenced for 386 genes associated with toxicological responses.In the pairwise comparison, 43 genes were differentially expressed upon exposure to indoor PM from damaged versus control homes; eight genes were differentially expressed more than five-fold. Both up- and down-regulation was demonstrated among the expressed genes. Most of the expressed genes were related to immunotoxicity; also genes related to steatosis, cholestasis, phospholipidosis and necrosis were affected. None of the genes was expressed systematically in all of the house pair -comparisons. The results indicate that the exposure to indoor PM from moisture-damaged houses activates especially immune defense-related genes in human airway constructs, but the effect does not manifest in the same way in every moisture-damaged house.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 4923.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).