PT - JOURNAL ARTICLE AU - Fabricio Santos AU - Francisca Lucas Froio AU - Gabriel Ribeiro Júnior AU - Mariana Matera Veras AU - Lisie Justo AU - Paulo Saldiva AU - Natalia De Souza Xavier Costa AU - Thais Mauad TI - Stereological approach to the pathology of lung aging in humans AID - 10.1183/13993003.congress-2020.1893 DP - 2020 Sep 07 TA - European Respiratory Journal PG - 1893 VI - 56 IP - suppl 64 4099 - http://erj.ersjournals.com/content/56/suppl_64/1893.short 4100 - http://erj.ersjournals.com/content/56/suppl_64/1893.full SO - Eur Respir J2020 Sep 07; 56 AB - Knowing lung structure during aging is important to understand the physiology of the senile process and related pathologies, such as COPD. There are scarce pathological data in humans.Aim: To assess, using stereology, lung volume and its compartments in adults and elderly human lungs.Methods: Ten autopsy right lungs from non-smokers, without lung pathology were obtained after ethical approval. Lungs were tracheally inflated with fixative under constant pressure of 20mmHg. Subsequently, the lobes were separated, and the total volumes were assessed by Cavalieri method. Lung tissue was sampled by Fractionator according to systematic uniform random sampling principles. Using H&E, we assessed absolute volume and volume density of airways, vessels, alveolar air spaces and septa by Cavalieri method.Results: The Adult Group (n=5) had mean age of 49.8y (46-59), 4M/1F. The Elderly Group (EG) (n=5) had mean age of 74.8y (60-87), 3M/2F. The EG had significantly whole right lung larger air space volume density (VD) (0.44±0.06vs0.52±0.02, p=0.018) and decreased alveolar septa VD (0.37±0.01vs0.32±0.03, p=0.016), also observed in the superior (p= 0.049, 0.021) and middle lobes (p=0.038 air spaces only). No differences were observed for right lung, lobes and compartment absolute volume (normalized by height). There was a positive correlation between age and air space VD (p=0.013, r=0.75) and a negative correlation with septa VD (p=0.034 r=-0.67).Conclusion: Although absolute lung volumes are not altered in EG, there is an altered VD of alveolar air spaces/septa, especially in the upper lobe, that correlates with age. Our stereological data in humans confirm previous experimental studies and radiological findings.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 1893.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).