RT Journal Article
SR Electronic
T1 Instant on-site histological analysis of fresh lung tumor tissue using third and second harmonic generation microscopy
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1640
DO 10.1183/13993003.congress-2020.1640
VO 56
IS suppl 64
A1 Laura Van Huizen
A1 Tess Kramer
A1 Danielle Seinstra
A1 Teodora Radonic
A1 Chris Dickhoff
A1 Hans Daniels
A1 Idris Bahce
A1 Peter Bonta
A1 Jouke Annema
A1 Marloes Groot
YR 2020
UL http://erj.ersjournals.com/content/56/suppl_64/1640.abstract
AB Introduction: In (suspected) lung cancer patients undergoing surgery, immediate feedback on the nature of the excised tissue and the surgical margin can be key in deciding the extent of the surgical resection. Third and second harmonic generation (THG/SHG) microscopy is a label-free, transportable, ex-vivo imaging technique that provides 3D images with subcellular details within seconds.Methods: In patients with suspected or proven lung cancer, samples of unprocessed tumor and healthy tissue were imaged with the THG/SHG microscope immediately after excision. Subsequently the tissue specimens were histologically analyzed and compared with the THG/SHG images.Results: In 4 patients healthy and tumor tissue (n=3 adenocarcinoma, n=1 carcinoid) was imaged with the THG/SHG microscope. Immediate high-quality images were obtained in all specimens. Malignancy could be detected as a disrupted alveolar pattern and an increased density of cellular structures with enlarged nuclei. Clear distinction between malignant and surrounding healthy tissue could be made.Conclusion: We present the first ex-vivo THG/SHG images, worldwide, of lung cancer tissue acquired with a mobile THG/SHG microscope. The very detailed images distinguish between healthy and malignant excised tissue. In the future, instant on-site histologic analysis using THG/SHG microscope might improve surgical/bronchoscopical outcomes. FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 1640.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).