RT Journal Article
SR Electronic
T1 Expression of the PDCD1 gene to determine active tuberculosis infection in children and adolescents with latent TB infection
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1563
DO 10.1183/13993003.congress-2020.1563
VO 56
IS suppl 64
A1 Mikhail Vladimirsky
A1 Andrey Elov
A1 Valentina Aksenova
A1 Kirill Gerasimov
YR 2020
UL http://erj.ersjournals.com/content/56/suppl_64/1563.abstract
AB Introduction: The prevalence of tuberculosis infection requires identifying groups of children and adolescents with a high risk of active tuberculosisAims and Objectives: To study the effectiveness of analysis of gene expression in blood cells to develop methods for determining the activity of tuberculosis infection in children and adolescents.Methods: We examined 24 children and adolescents from 6 to 16 years old with active pulmonary tuberculosis, of which in only one case was the release of mycobacterium tuberculosis. Another group examined 36 children and adolescents with latent tuberculosis infection, established according to the results of a specific skin test Diaskin-test, based on the use of MTB protein ESAT-6-CFP-10. The expression of 7 genes in blood cells was analyzed: GADPH (control gene), ASUN, NEMF, PTRC, NPC2, PDCD1, and PD-L1. Isolation of mRNA was performed from 100 μl of blood using Trizol LS Reagent (Ambion) according to the manufacturer's instructions, but with the extraction of RNA from the aqueous phase on a magnetic sorbent (Syntol, Russia). RNA-PCR was performed using the appropriate primers for each gene and the Power Sybr Green RNA-to-Ct 1-Step kit (Applied Biosystems). The results were analyzed using statistical programs PRISM 4 and ROC analysis.Results: The most significant differences between groups with active and latent infection there was the results of the analysis of the PDCD1 gene: sensitivity -95.8%, specificity 94.4%, area under the curve - 0.96 (Fig.1), P<0,0001.Conclusions: PDCD1 gene expression analysis may be a promising method for determining the prognosis of tuberculosis infection activity.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 1563.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).