RT Journal Article SR Electronic T1 IgE-mediated inflammatory response induced by Crotalus durissus venom in mice JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP 1982 DO 10.1183/13993003.congress-2020.1982 VO 56 IS suppl 64 A1 Ricardo Figueiredo A1 Marcos Lázaro Guerreiro A1 Nataly De Oliveira A1 Elen Azevedo A1 Paulo Cesar Nunes A1 Neuza Alcantara-Neves A1 Ilka Biondi YR 2020 UL http://erj.ersjournals.com/content/56/suppl_64/1982.abstract AB Neurotoxic events have been recognized as the main clinical manifestation in Crotalus durissus accidents. Anaphylactic shock is a life-threatening medical condition although reports of hypersensitivity reactions to snake venom are limited.The aim of this study was to evaluate IgE-mediated inflammation in C. durissus venom response. The experiments were performed using 22 mice weighing 20-28 grams challenged intraperitoneally (i.p) on the 1st and 14th days. Each group received (i) 50% DL50% (0.25µg/kg) venom with aluminum hydroxide (AlOH3), (ii) AlOH3 solution or (iii) saline solution (PBS). On the 21st day, experiment group was exposed via aerosol either to 5 DL50% (5 µg) or 10 DL50% (10 µg), while control group was nebulized with PBS. The animals were euthanized after 24 hours. Serum IgE antibodies were detected by ELISA and lung fragments were collected for histological analysis.The experimental group developed erythema, respiratory distress, tremors and pruritus in the nasal region and at the inoculation site. Serum levels of IgE were statistically significant higher in animals previous exposed to venom (Figure 1) and pulmonary parenchyma exhibited septal inflammation with macrophages distributed along the alveolar wall.These findings advance our understanding of pathophysiological pathways and the role of allergenic proteins in hypersensitivity reaction following exposure to C. durissus venom.FootnotesCite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 1982.This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).