TY - JOUR T1 - International consensus guideline for reporting transmission electron microscopy results in the diagnosis of primary ciliary dyskinesia (BEAT PCD TEM Criteria) JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.00725-2019 VL - 55 IS - 4 SP - 1900725 AU - Amelia Shoemark AU - Mieke Boon AU - Christoph Brochhausen AU - Zuzanna Bukowy-Bieryllo AU - Maria M. De Santi AU - Patricia Goggin AU - Paul Griffin AU - Richard G. Hegele AU - Robert A. Hirst AU - Margaret W. Leigh AU - Alison Lupton AU - Karen MacKenney AU - Heymut Omran AU - Jean-Claude Pache AU - Andreia Pinto AU - Finn P. Reinholt AU - Josep Schroeder AU - Panayotis Yiallouros AU - Estelle Escudier A2 - , Y1 - 2020/04/01 UR - http://erj.ersjournals.com/content/55/4/1900725.abstract N2 - Primary ciliary dyskinesia (PCD) is a heterogeneous genetic condition. European and North American diagnostic guidelines recommend transmission electron microscopy (TEM) as one of a combination of tests to confirm a diagnosis. However, there is no definition of what constitutes a defect or consensus on reporting terminology. The aim of this project was to provide an internationally agreed ultrastructural classification for PCD diagnosis by TEM.A consensus guideline was developed by PCD electron microscopy experts representing 18 centres in 14 countries. An initial meeting and discussion were followed by a Delphi consensus process. The agreed guideline was then tested, modified and retested through exchange of samples and electron micrographs between the 18 diagnostic centres.The final guideline a) provides agreed terminology and a definition of Class 1 defects which are diagnostic for PCD; b) identifies Class 2 defects which can indicate a diagnosis of PCD in combination with other supporting evidence; c) describes features which should be included in a ciliary ultrastructure report to assist multidisciplinary diagnosis of PCD; and d) defines adequacy of a diagnostic sample.This tested and externally validated statement provides a clear guideline for the diagnosis of PCD by TEM which can be used to standardise diagnosis internationally.This tested and validated consensus statement provides clear terminology and defect definitions for the ultrastructural diagnosis of primary ciliary dyskinesia (PCD). The guideline will facilitate standardisation of PCD research and diagnosis. http://bit.ly/2RDkz7k ER -