TY - JOUR T1 - Bronchoscopic brushing from central lung cancer – Next generation sequencing results are reliable JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2019.PA3081 VL - 54 IS - suppl 63 SP - PA3081 AU - Lars Hagmeyer AU - Jana Fassunke AU - Marianne Engels AU - Marcel Treml AU - Simon Herkenrath AU - Sandhya Matthes AU - Reinhard Büttner AU - Winfried Randerath Y1 - 2019/09/28 UR - http://erj.ersjournals.com/content/54/suppl_63/PA3081.abstract N2 - The role of bronchoscopic brushing for tumor detection and molecular testing in central lung cancer is unclear.In this study, 50 consecutive subjects with suspected central lung cancer underwent bronchoscopic brushing (31 male, median age 70, 5 never smokers). Histological results were: NSCLC/SCLC/low-grade-NET/granulation tissue in 36/8/2/4 cases.Next Generation Sequencing (NGS) was feasible in 62% of tumor-positive brush smear samples. In 78% of these cases NGS displayed identical results compared to histology samples, in 22% NGS from brush smears detected specific mutations whereas DNA quality from forceps biopsy was insufficient for NGS analysis. Sensitivity, specificity, positive predictive value, negative predictive value of brush smear analysis were 66% (95% confidence interval 50-79), 100% (40-100), 100% (85-100), and 21% (7-46). For the combined analysis of brush smear, brush tip washing and sheath tube content sensitivity was slightly elevated at 69% (53-81).In central lung cancer, bronchoscopic brushing detects tumor cells in about two third of cases and allows a decision for or against targeted therapy in the majority of tumor-positive cases on the basis of NGS analysis.FootnotesCite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA3081.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only). ER -