RT Journal Article
SR Electronic
T1 Bronchoscopic brushing from central lung cancer – Next generation sequencing results are reliable
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA3081
DO 10.1183/13993003.congress-2019.PA3081
VO 54
IS suppl 63
A1 Lars Hagmeyer
A1 Jana Fassunke
A1 Marianne Engels
A1 Marcel Treml
A1 Simon Herkenrath
A1 Sandhya Matthes
A1 Reinhard Büttner
A1 Winfried Randerath
YR 2019
UL http://erj.ersjournals.com/content/54/suppl_63/PA3081.abstract
AB The role of bronchoscopic brushing for tumor detection and molecular testing in central lung cancer is unclear.In this study, 50 consecutive subjects with suspected central lung cancer underwent bronchoscopic brushing (31 male, median age 70, 5 never smokers). Histological results were: NSCLC/SCLC/low-grade-NET/granulation tissue in 36/8/2/4 cases.Next Generation Sequencing (NGS) was feasible in 62% of tumor-positive brush smear samples. In 78% of these cases NGS displayed identical results compared to histology samples, in 22% NGS from brush smears detected specific mutations whereas DNA quality from forceps biopsy was insufficient for NGS analysis. Sensitivity, specificity, positive predictive value, negative predictive value of brush smear analysis were 66% (95% confidence interval 50-79), 100% (40-100), 100% (85-100), and 21% (7-46). For the combined analysis of brush smear, brush tip washing and sheath tube content sensitivity was slightly elevated at 69% (53-81).In central lung cancer, bronchoscopic brushing detects tumor cells in about two third of cases and allows a decision for or against targeted therapy in the majority of tumor-positive cases on the basis of NGS analysis.FootnotesCite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA3081.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).