PT - JOURNAL ARTICLE AU - Irina Ponomareva AU - Nailya Asfandiyarova AU - Dmitriy Kuzmin TI - Effect of nicotine on the system of cell-mediated immunity in COPD AID - 10.1183/13993003.congress-2019.PA4388 DP - 2019 Sep 28 TA - European Respiratory Journal PG - PA4388 VI - 54 IP - suppl 63 4099 - http://erj.ersjournals.com/content/54/suppl_63/PA4388.short 4100 - http://erj.ersjournals.com/content/54/suppl_63/PA4388.full SO - Eur Respir J2019 Sep 28; 54 AB - Smoking is the leading cause of COPD and mortality from it. Effects of smoking on various cells is well known, however, there is no consensus of opinion on its effect on the immune system. The main aim of our investigation was the study of nicotine effect on cell-mediated immunity (CMI) in patients with COPD.Material and methods: the study included 26 patients with COPD, the index of pack-years (IPY) 26.7 ± 7.3 and 25 patients of the control group. CMI was estimated by proliferative activity of lymphocytes in response to mitogen (phytohemagglutinin, PHA) and nicotine (1:1000).Results: CMI in groups B, C and D of COPD patients was decreased (49.5±4.8%, vs. 66.7±3.4% p<0.001), however, in group A of COPD patients, the decrease does not reach statistically significant values (57.0±4.9% vs. 66.7±3.4%, p>0.05). CMI depended on the duration of smoking and the number of smoked cigarettes (IPY< 30 proliferative activity to PHA: 52.9±3,4%, IPY >30: 43,5±1,8%, p<0.05). Nicotine did not cause significant changes in the level of lymphocyte proliferation in the control cell culture (with saline). The addition of nicotine together with PHA to the cell culture was associated with suppression of CMI in 67 % of COPD patient and control group. Stimulation of proliferative activity of lymphocytes was revealed in 33% of the investigated persons.Conclusion: long-term experience of smoking causes a decrease in the activity of cell-mediated immunity in the majority of patients. These and following studies will create an informed basis for the working out of targeted therapy and targeted prevention methodsFootnotesCite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA4388.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).