RT Journal Article
SR Electronic
T1 Hyperinsulinemia due to altered insulin secretion contributes to insulin resistance in chronic intermittent hypoxia independently of obesity
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP OA4935
DO 10.1183/13993003.congress-2019.OA4935
VO 54
IS suppl 63
A1 Joana F. Sacramento
A1 Bernardete F. Melo
A1 Elena Olea
A1 Ana Obeso
A1 Asuncion Rocher
A1 Tiago Rodrigues
A1 Jesus Prieto-Lloret
A1 Sara Yubero
A1 Emilia C. Monteiro
A1 Paulo Matafome
A1 Silva V. Conde
YR 2019
UL http://erj.ersjournals.com/content/54/suppl_63/OA4935.abstract
AB Clinical studies have demonstrated a link between obstructive sleep apnea (OSA) and insulin resistance (IR), however it is not clear which is the main event that determines IR development. We hypothesize that hyperinsulinemia is a main driver of IR in chronic intermittent hypoxia (CIH), that mimics OSA, independently of obesity, adipose tissue hypoxia and severity of CIH.Male Wistar Rats were submitted to 2 CIH protocols: 1) a mild CIH paradigm obtained by 5/6 hypoxic (5%O2) cycles/h, 10.5 h/day during 35 days; and 2) a severe CIH paradigm obtained by 40 s, 5%O2/80 s, air, equivalent to an apnoea–hypopnoea index of 30, 8h/day for 14 days. CIH animals were compared with controls. Fasting glycemia, insulinemia, C-peptide and insulin sensitivity through KITTand HOMA index were measured. Insulin clearance was calculated. Weight and fat mass were assessed. Adipose tissue dysfunction and inflammation were assessed by evaluation of several proteins involved in insulin signaling pathways and hypoxia and inflammation pathways.Mild CIH increased insulin secretion by 214% (CTLmildCIH =1.01±0.21ug/L) while severe CIH increased by 103% (CTLsevereCIH= 1.1±0.04ug/L) with no alterations in fasting glycemia in both CIH protocols. HOMA index increased by 112% in mild CIH and by 108% in severe CIH animals. None of CIH protocols alter weight gain and fat mass deposition. Mild and severe CIH increased IR in adipose tissue, with no changes in HIF1 and 2α expression.We can conclude that both mild and severe CIH protocols induce insulin resistance, which is associated with an increase insulin secretion by the pancreas being independent of obesity and fat mass.FootnotesCite this article as: European Respiratory Journal 2019; 54: Suppl. 63, OA4935.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).