RT Journal Article
SR Electronic
T1 Role of Inducible bronchial-associated lymphoid tissue (iBALT) in airway remodelling of murine chronic asthma model
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP OA1629
DO 10.1183/13993003.congress-2019.OA1629
VO 54
IS suppl 63
A1 Aya Watanabe
A1 Atsuyasu Sato
A1 Tatsushi Mizutani
A1 Yoko Hamakawa
A1 Kiyoshi Uemasu
A1 Toyohiro Hirai
YR 2019
UL http://erj.ersjournals.com/content/54/suppl_63/OA1629.abstract
AB Introduction: iBALT is a tertiary lymphoid tissue developed in infectious, auto-immune and inflammatory lung diseases. In asthma, iBALT formation is often found in lung, but its function is not fully investigated.Object: To evaluate the function of iBALT in chronic asthma using murine model.Methods: Since Cxcl13 is a critical chemokine to induce iBALT by stimulating Cxcr5 on Tfh cells, B cells, macrophages and DCs, Cxcl13 KO mice and control mice were exposed to house dust mite antigen (HDMA) for 7wks to develop chronic asthma phenotypes. Immune cell proportion and numbers in BALF were analysed by flow cytometry. Histological analysis including morphometry, immunohistochemistry and immune-fluorescence study were performed. mRNA expression in lung was evaluated by qRT-PCR.Result: There was no difference in the proportion of eosinophils in BALF between Cxcl13 KO mice (mean 58.2%; SE 5.7%) and control mice (mean 59.7%; SE 4.6%) after HDMA treatment, suggesting no change in Th2 pathway. In the tissue of chronic HDMA-exposed control mice, we found multiple iBALT formations adjacent to bronchi. Significant secondary lymph node swelling around trachea was also observed only in control mice. On the other hand, Cxcl13 KO mice showed T-B cell infiltration, but iBALT formation was almost completely diminished. Finally, significantly less thickening of airway was observed in Cxcl13 KO mice compared to that in control mice, supporting the previous evidence that B cell stimulation is critical for airway remodelling rather than eosinophilic inflammation.Conclusion: iBALT might contribute to airway remodelling in asthma independent of eosinophilic inflammation.FootnotesCite this article as: European Respiratory Journal 2019; 54: Suppl. 63, OA1629.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).