PT  - JOURNAL ARTICLE
AU  - JongWook Shin
AU  - Ho Jeong Kim
AU  - Byoung Whui Choi
AU  - In Won Park
TI  - Autophagic and apoptotic changes in macrophages in response to graphene oxides with or without chemical modification
AID  - 10.1183/13993003.congress-2019.PA2439
DP  - 2019 Sep 28
TA  - European Respiratory Journal
PG  - PA2439
VI  - 54
IP  - suppl 63
4099  - http://erj.ersjournals.com/content/54/suppl_63/PA2439.short
4100  - http://erj.ersjournals.com/content/54/suppl_63/PA2439.full
SO  - Eur Respir J2019 Sep 28; 54
AB  - Fine dust particles can derived from desert and industrial derivatives. The graphene, an allotrope of carbon, has the honeycombing structure of one-atom-thick planar sheets, is widely used for the modern electronics, informative technologies including medical devices. It may provoke various immune responses. We tested the autophagic and apoptotic response of macrophages in response to graphene and its chemically modified compounds. Raw264.7 cells were treated with graphene oxide(GO), reduced GO(RGO), SDS-GO, dodecyl amine(DA)-GO with different concentrations and times. MTT assay and Western blotting for PU.1, mTOR, NBR1, p62, Beclin-1 and LC3-A/B-I/II. The cell viability was reduced after treatment of 4 kinds of GO compounds in macrophages in light microscopy and MTT assay. PU.1 decrease was more remarkable in response to treatment of GO compounds- dose dependent manner than R-, DA- and SDS-GO. LC3-A/B-I to LC3A/B-II conversion was dose-dependently decreased after treatment of four GO’s. The expressional levels of mTOR and NBR1 showed similar manner in GO but not remarkable in other three compounds.P62 and Bec-1 were not remarkably changed in four GO compounds. Although the cell viability decreased in GO-treated macrophages, autophagy, apoptosis and PU.1 decreased in higher concentrations of GO compounds. These findings may suggest the other important complicated mechanisms of cellular injury in macrophages. Further evaluation may be necessary.FootnotesCite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA2439.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).