TY - JOUR T1 - Mycobacterium tuberculosis detection rates by molecular genetic methods among patients with tuberculosis in the Russian Federation JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2019.PA2991 VL - 54 IS - suppl 63 SP - PA2991 AU - Elena Veselova AU - Anna Panova AU - Grigory Kaminskiy AU - Vadim Testov AU - Anastasia Samoilova AU - Irina Vasilyeva Y1 - 2019/09/28 UR - http://erj.ersjournals.com/content/54/suppl_63/PA2991.abstract N2 - Introduction: Molecular genetic methods (MGM) provide rapid detection of Mycobacterium tuberculosis (MTB) DNA and DST. It is critical important for TB and HIV co-infected patients due to acute and severe TB manifestation.Aims: To study efficiency of MTB DNA detection (MTBD) by MGM among new TB cases with HIV positive (HIV+) and HIV negative (HIV-) status.Methods: 9 TB hospitals from different Russian regions were randomly selected and 433 new TB cases, detected in 2018, were included in study: 115 HIV+, 318 HIV- patients. We used real-time PCR (Amplitub-qPCR) for MTBD. Detection rate was defined as the proportion of cases, verified by PCR to all included TB cases.Results: Clinical manifestations of TB were: pulmonary tuberculosis without cavity (PTB) (235 cases), PTB with cavity (PTBC) (175 cases), extrapulmonary TB (EPTB) (23 cases).Proportion of TB clinical manifestations in HIV+ patients:EPTB (5.2%) <PTBC (27.0%) <PTB (67.8%)Proportion of TB clinical manifestations in HIV- patients:EPTB (5.3%) <PTBC (45.3%) <PTB (49.4%)Table. The efficiency of MTBD depending on HIV-status and clinical manifestations (%).View this table:Conclusion: The efficiency of MTBD by used PCR systems comprised 73.7%. There was high efficiency of MTBD for new TB cases with HIV+ and HIV- status. The efficiency of MTBD for new EPTB cases with HIV- was fairly high – 58.8%.FootnotesCite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA2991.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only). ER -