RT Journal Article
SR Electronic
T1 Eosinophils promote airway smooth muscle cells differentiation to the contractile phenotype in asthma
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA5208
DO 10.1183/13993003.congress-2019.PA5208
VO 54
IS suppl 63
A1 Ieva Janulaityte
A1 Andrius Januskevicius
A1 Virginija Kalinauskaite-Zukauske
A1 Reinoud Gosens
A1 Airidas Rimkunas
A1 Greta Gabuzeviciute
A1 Kestutis Malakauskas
YR 2019
UL http://erj.ersjournals.com/content/54/suppl_63/PA5208.abstract
AB Background: ASM cells can switch between a synthetic-proliferative and a contractile phenotype. Contractile ASM cells phenotype is described by contractile apparatus proteins expression but the role of eosinophils (EOS) in ASM phenotype switch is unknown.Aim: To evaluate the impact of EOS on ASM cells differentiation to contractile phenotype in asthma.Methods: 7 allergic asthma(AA) and 7 healthy subjects(HS)were included. AA patients additionaly underwent bronchial allergen challenge. Peripheral blood was collected before and 24h after the challenge. Blood EOS were isolated using high-density centrifugation and magnetic separation. Individual co-cultures of EOS and hASM cell line were prepared. Gene expression was evaluated using qPCR analysis, contractility was evaluated after 24h using collagen gel.Results: Gene expression of α-sm-actin,sm-MHC,SM22, calponin,sm-MLCK was increased in ASM cells after incubation with AA EOS compared to HS, respectively 1.2±0.1vs.0.9±0.1; 3.3±0.6vs.1.3±0.5; 3.3±1.0vs.0.8±0.2; 1.3±0.3vs.0.5±0.1; 3.4±0.7vs.1.3±05 folds over control,p<0.05. Further, in vivo allergen-activated EOS enhanced gene expression of α-sm-actin,sm-MHC,SM22,sm-MLCK compared to the AA group baseline(respectively 2.3±0.5vs.1.2±0.1; 7.3±1.6vs.3.3±0.6; 9.6±2.7vs.3.3±1.0; 8.3±2.6vs. 3.4±0.7, p<0.05), however, did not affect calponin gene expression. AA EOS significantly increased ASM cells contractility compared to HS EOS,13.7±1vs.6.9±2% of control,p<0.05, when allergen-activated EOS further increased contractility compared to non-activated AA EOS(23.6±4 vs. 13.7±1% of control,p<0.05).Conclusion: Asthmatic EOS, particularly, allergen-activated, promoted ASM cells differentiation to contractile phenotype.FootnotesCite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA5208.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).