PT  - JOURNAL ARTICLE
AU  - Małgorzata Proboszcz
AU  - Patrycja Nejman-Gryz
AU  - Magdalena Paplińska-Goryca
AU  - Katarzyna Górska
AU  - Paulina Misiukiewicz-Stępień
AU  - Rafał Krenke
TI  - Expression of CCR3 on sputum macrophages in asthma, COPD and healthy subjects
AID  - 10.1183/13993003.congress-2019.PA4085
DP  - 2019 Sep 28
TA  - European Respiratory Journal
PG  - PA4085
VI  - 54
IP  - suppl 63
4099  - http://erj.ersjournals.com/content/54/suppl_63/PA4085.short
4100  - http://erj.ersjournals.com/content/54/suppl_63/PA4085.full
SO  - Eur Respir J2019 Sep 28; 54
AB  - Macrophages are important immune cells involved in airway inflammation in obstructive lung diseases. Depending on local milieu macrophages can undergo distinct polarization with different functional capacities. Expression of CCR3 receptor on macrophages is associated with Th2 response but its role in Th1 response remains unclear.The aim was to evaluate the expression of CCR3 receptor on M1- and M2-like sputum macrophages in obstructive lung diseases and healthy controls.Study involved 19 asthmatics, 21 COPD patients, 9 healthy non-smokers, and 9 healthy smokers who underwent sputum induction. In immunofluorescence staining, CCR3 expression was assessed on CD80/CD86 positive (M1) and CD163/CD206 positive (M2) macrophages in sputum smears. Non-smokers and smokers were comparators for asthma and COPD, respectively. We noted increased number of M1+CCR3+ and M2+CCR3+ sputum macrophages in asthma vs non-smoking controls, in smoking control vs non-smoking control and in smoking control vs COPD.View this table:Conclusion: Our study demonstrated an enhanced expression of CCR3 on M1 and M2 macrophages in asthma, COPD patients and healthy smokers. It suggests that expression of CCR3 on macrophages may be associated not only with Th2 response but also with Th1 response and be influenced by cigarette smoke exposure.FootnotesCite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA4085.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).