TY - JOUR T1 - Exosomes form EGFR-mutated adenocarcinoma induce tumour invasion JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2019.PA3670 VL - 54 IS - suppl 63 SP - PA3670 AU - Amina Jouida AU - Elisabetta Crisetti AU - Cormac Mccarthy AU - Aurelie Fabre AU - Karl Mcauley AU - Alan Kelly AU - Marissa Ocallaghan AU - Michael Keane Y1 - 2019/09/28 UR - http://erj.ersjournals.com/content/54/suppl_63/PA3670.abstract N2 - Exosomes are a class of extra cellular vesicles (EVs), with a multi vesicular endosomal origin, that are released by all cell types, with sizes ranging from 30-100nm and a lipid bilayer membrane. Exosomes and their contents have emerged as a potential source of information on tumour detection and regulatory drivers of tumour progression and metastasis. They have been reported to influence epithelial mesenchymal transition (EMT) in pathological states such as metastatic lung cancer. We analysed the effects of exosomes derived from serum of different lung cancer patients: Epidermal Growth Factor Receptor (EGFR)-mutated adenocarcinoma (N=6), wild-type adenocarcinoma (N=3), squamous (N=1) and serum of patient with no cancer (N=1) on cells from an A549 cell line.We observed by zymography, that all exosomes, regardless of patient origin, induced an increase in MMP2 activity in all A549 cells treated compared to the non-treated cells. However, the increase of pro-MMP9 and MMP9 activity was seen only in A549 cells treated with exosomes derived from EGFR-mutated adenocarcinoma patients (respectively a fold increase of 54 and 37 compared to the non-treated cells).MMPs are one of the major attributes that epithelial cells acquire after undergoing EMT. Our results suggest that exosomes derived from EGFR-mutated adenocarcinoma serum could be a potential mediator of EMT and tumour invasion and may provide insights into the mechanisms of lung cancer progression and metastasis. However, additional studies are required to precisely delineate these mechanisms.FootnotesCite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA3670.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only). ER -