RT Journal Article SR Electronic T1 An RNA-Seq Primer for Pulmonologists JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP 1801625 DO 10.1183/13993003.01625-2018 A1 Sarah G. Chu A1 Sergio Poli De Frias A1 Benjamin A. Raby A1 Ivan O. Rosas YR 2019 UL http://erj.ersjournals.com/content/early/2019/09/19/13993003.01625-2018.abstract AB With the evolution of high throughput sequencing technologies, the past decade has seen an exponential rise in the use of RNA sequencing (RNA-seq). RNA-seq has deepened our understanding of biological systems to unprecedented levels of resolution, identifying not only gene expression signatures but also regulatory RNA molecules that may play critical roles in disease pathogenesis. Pulmonary research has quickly incorporated this technology, from characterizing the IL-17 signature of steroid-unresponsive COPD patients [1] to discovering pathogen-host interactions of M. tuberculosis [2]. This mini-review provides an overview of core features and applications of RNA-seq to familiarize non-experts with the methodology and how it has impacted our understanding of lung pathophysiology.FootnotesThis manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.Conflict of interest: Dr. Chu has nothing to disclose.Conflict of interest: Dr. Raby reports grants from National Institutes of Health, during the conduct of the study.Conflict of interest: Dr. Rosas reports grants from National Institutes of Health, during the conduct of the study.Conflict of interest: Dr. Poli has nothing to disclose.