RT Journal Article
SR Electronic
T1 Bacterial load and defective monocyte-derived macrophage bacterial phagocytosis in biomass smoke-related COPD
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1702273
DO 10.1183/13993003.02273-2017
VO 53
IS 2
A1 Baishakhi Ghosh
A1 Akshay H. Gaike
A1 Kanchan Pyasi
A1 Bill Brashier
A1 Vandana V. Das
A1 Jyoti D. Londhe
A1 Sanjay Juvekar
A1 Yogesh S. Shouche
A1 Louise E. Donnelly
A1 Sundeep S. Salvi
A1 Peter J. Barnes
YR 2019
UL http://erj.ersjournals.com/content/53/2/1702273.abstract
AB Lower airway colonisation with species of potentially pathogenic bacteria (PPB) is associated with defective bacterial phagocytosis, in monocyte-derived macrophages (MDMs) and alveolar macrophages, from tobacco smoke-associated chronic obstructive pulmonary disease (S-COPD) subjects. In the developing world, COPD among nonsmokers is largely due to biomass smoke (BMS) exposure; however, little is known about PPB colonisation and its association with impaired innate immunity in these subjects.We investigated the PPB load (Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Pseudomonas aeruginosa) in BMS-exposed COPD (BMS-COPD) subjects compared with S-COPD and spirometrically normal subjects. We also examined the association between PPB load and phagocytic activity of MDMs and lung function. Induced sputum and peripheral venous blood samples were collected from 18 healthy nonsmokers, 15 smokers without COPD, 16 BMS-exposed healthy subjects, 19 S-COPD subjects and 23 BMS-COPD subjects. PPB load in induced sputum and MDM phagocytic activity were determined using quantitative PCR and fluorimetry, respectively.Higher bacterial loads of S. pneumoniae, H. influenzae and P. aeruginosa were observed in BMS-COPD subjects. Increased PPB load in BMS-exposed subjects was significantly negatively associated with defective phagocytosis in MDMs and spirometric lung function indices (p<0.05).Increased PPB load in airways of BMS-COPD subjects is inversely associated with defective bacterial phagocytosis and lung function.Bacterial load in biomass smoke-exposed COPD subjects is associated with defective macrophage bacterial phagocytosis http://ow.ly/Q5ns30mNN0k