TY - JOUR T1 - Use of three-dimensional organoids and lung-on-a-chip methods to study lung development, regeneration and disease JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.00876-2018 VL - 52 IS - 5 SP - 1800876 AU - Konstantinos Gkatzis AU - Sara Taghizadeh AU - Dongeun Huh AU - Didier Y.R. Stainier AU - Saverio Bellusci Y1 - 2018/11/01 UR - http://erj.ersjournals.com/content/52/5/1800876.abstract N2 - Differences in lung anatomy between mice and humans, as well as frequently disappointing results when using animal models for drug discovery, emphasise the unmet need for in vitro models that can complement animal studies and improve our understanding of human lung physiology, regeneration and disease. Recent papers have highlighted the use of three-dimensional organoids and organs-on-a-chip to mimic tissue morphogenesis and function in vitro. Here, we focus on the respiratory system and provide an overview of these in vitro models, which can be derived from primary lung cells and pluripotent stem cells, as well as healthy or diseased lungs. We emphasise their potential application in studies of respiratory development, regeneration and disease modelling.3D organoids and lung-on-a-chip: modelling lung physiology and pathology in a dish http://ow.ly/NWCN30m0SyJ ER -