RT Journal Article
SR Electronic
T1 Bacterial load and defective monocyte-derived macrophage bacterial phagocytosis in biomass-smoke COPD
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1702273
DO 10.1183/13993003.02273-2017
A1 Baishakhi Ghosh
A1 Akshay H. Gaike
A1 Kanchan Pyasi
A1 Bill Brashier
A1 Vandana V. Das
A1 Jyoti D. Londhe
A1 Sanjay Juvekar
A1 Yogesh S. Shouche
A1 Louise E. Donnelly
A1 Sundeep S. Salvi
A1 Peter J. Barnes
YR 2018
UL http://erj.ersjournals.com/content/early/2018/11/21/13993003.02273-2017.abstract
AB Lower airway colonisation with potentially pathogenic bacterial species (PPBs) is associated with defective bacterial phagocytosis, in monocyte-derived macrophages (MDMs) and alveolar macrophages, from tobacco-smoke associated COPD (S-COPD) subjects. In developing world, COPD among non-smokers is largely due to biomass-smoke (BMS) exposure. Yet, little is known about PPBs colonisation and its association with impaired innate immunity in these subjects.We investigated the PPBs load (Streptococcus pneumoniae, SP; Haemophilus influenzae, HI; Moraxella catarrhalis, MC; and Pseudomonas aeruginosa, PA) in BMS-exposed COPD (BMS-COPD) compared with S-COPD and spirometrically normal subjects. We also examined the association between load of PPBs with phagocytic activity of MDMs and lung function.Induced sputum and peripheral venous blood samples were collected from 18 healthy non-smokers, 15 smokers without COPD, 16 BMS-exposed healthy, 19 S-COPD and 23 BMS-COPD subjects. PPBs load in induced sputum and MDMs phagocytic activity were determined using qPCR and fluorimetry respectively.Higher bacterial load of SP, HI, and PA were observed in BMS-COPD. Increased PPBs load in BMS-exposed subjects was significantly negatively associated with defective phagocytosis in MDMs, and spirometric lung function indices (p<0.05).Increased load of PPBs in airways of BMS-COPD subjects is inversely associated with defective bacterial phagocytosis and lung function.FootnotesThis manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.Conflict of interest: Dr. Ghosh has nothing to disclose.Conflict of interest: Dr. Gaike has nothing to disclose.Conflict of interest: Dr. Pyasi has nothing to disclose.Conflict of interest: Dr. Brashier has nothing to disclose.Conflict of interest: Dr. Das has nothing to disclose.Conflict of interest: Dr. Londhe has nothing to disclose.Conflict of interest: Dr. Juvekar has nothing to disclose.Conflict of interest: Dr. Donnelly reports grants from Cempra, grants from AstraZeneca, grants from Boehringer Ingelheim, outside the submitted work.Conflict of interest: Dr. Salvi has nothing to disclose.Conflict of interest: Dr. Barnes has nothing to disclose.Conflict of interest: Dr. Shauche has nothing to disclose.