RT Journal Article SR Electronic T1 Compliance with recommendation for respiratory sampling in diagnosis of Tuberculosis(NICE guideline NG33) JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP PA4760 DO 10.1183/13993003.congress-2018.PA4760 VO 52 IS suppl 62 A1 Aung, Hnin Wint Wint A1 Meghjee, Salim P L A1 Wearmouth, Debbie YR 2018 UL http://erj.ersjournals.com/content/52/suppl_62/PA4760.abstract AB Introduction and Objective: Tuberculosis is a global disease and detection of acid fast bacilli in sputum smears remains the most widely used rapid diagnostic test.NICE guideline recommended collection of multiple respiratory samples(3 deep cough sputa)for laboratory diagnosis of respiratory TB. We explored compliance against the guideline locally regarding submission of specimen for diagnosis.Method: A retrospective review of all sputum specimens for culture received by microbiology laboratory over 6 months(1/4/2016 to 30/09/2016) was done and broncho-alveolar lavage samples submitted in the same period were excluded as single BAL will suffice as per the guidance.Results: Receiving samples(n=526) from 245patients, overall compliance with guideline is only 43% with further 10% submitting 2 specimens. Each area of high demand, respiratory department and GP, achieved only 58.4% and 38.6% and the worst outcome is revealed in acute clinical care and non-respiratory specialities. Percentage of positive results increased from 0.88%(1 out of 113)with one sample to 6.6%(7 out of 106)in three samples.Compliance with guideline for sample request by departmentsConclusion: The project addressed the requirement to submit optimal number of sample request for good quality of diagnosis and it has led hospital IT system to automatically include 3 sputum sample requests for investigation of respiratory TB including access from GP.FootnotesCite this article as: European Respiratory Journal 2018 52: Suppl. 62, PA4760.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).