TY - JOUR T1 - MCC-IMS Spectral Analyses of Volatile Markers for Screening of Clinically Relevant Bacterial Infection JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.congress-2018.PA2615 VL - 52 IS - suppl 62 SP - PA2615 AU - Gunther Becher AU - Roman Purkhart AU - Romy Gerber AU - Werner Schueler AU - Rolf Graupner Y1 - 2018/09/15 UR - http://erj.ersjournals.com/content/52/suppl_62/PA2615.abstract N2 - Introduction: The detection of bacterial growth in culture last up to 24 h. Faster methods are cost expensive. Rapid and inexpensive methods for bacterial screening are needed. Ion Mobility Spectrometry (IMS) is a sensitive method for detection of volatile organic compounds (VOCs) in head space from bacterial cultures, able to give immediate result. Based on these findings the question remains whether bacterial VOC are detectable in exhaled breath. The study was aimed to check whether IMS is suitable as a screening for bacterial infections.Methods: Patients in a reception of a hospital were selected for an IMS analysis by single breath test. The VOC-dependent peaks in IMS-spectra were determined and compared by means of cluster analysis-based software. The classification has been carried out with a leave-one-out cross validation and support vector machine [1].Results: In total up to about 1581 different clusters were identified in breath samples. It was shown that at least five different clusters were suitable for differentiation of respiratory and non-respiratory infections.Conclusions: The distinction of an unknown bacterial infection by different species with 100% sensitivity was possible, the specifity for correct exclusion of infection was about 80%.The method enables rapid screening for infectious diseases as well as other metabolic disorders [2]. The rate of false positive results is acceptable, also keeping in mind that a negative bacteriological finding with probative force seems not possible.Ref.:[1] R. Purkhart et al.: J. Breath Res. 5 (2011) 027103 (10pp)[2] G. Becher et al.: archive euromedica 1 (2015) 3-5FootnotesCite this article as: European Respiratory Journal 2018 52: Suppl. 62, PA2615.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only). ER -