RT Journal Article
SR Electronic
T1 Cigarette smoke upregulating the expression of miR 21 increases IL-8 in airway epithelial cells
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA2171
DO 10.1183/13993003.congress-2018.PA2171
VO 52
IS suppl 62
A1 Elisabetta Pace
A1 Serena Di Vincenzo
A1 Eleonora Di Salvo
A1 Maria Ferraro
A1 Paola Dino
A1 Federico Saibene
A1 Luigi Lanata
A1 Sebastiano Gangemi
YR 2018
UL http://erj.ersjournals.com/content/52/suppl_62/PA2171.abstract
AB Cigarette smoke exposure in airway epithelial cells increasing both TLR4 and ROS expression promotes IL-8 mediated inflammatory responses. Since expression of TLR4 is correlated with miR-21 expression and ROS production in human lung cancer cells, the present study was aimed to explore whether cigarette smoke exposure, altering miR-21 expression, promoted inflammatory responses in airway epithelial cells. Airway epithelial cells (16HBE and A549) were exposed to cigarette smoke extracts (CSE) 10% and 20% and/or with/without agomiR-21, apigenin and carbocysteine and then it was assessed: miR-21 expression by Real Time PCR; pERK1/2/tERK1/2 ratio by western blot analysis; IL-8 mRNA by Real Time PCR and IL-8 protein release by ELISA.It was demonstrated that, in both cell lines, CSE exposure and agomiR-21 increased: miR-21 expression; pERK1/2/tERK1/2 ratio; IL-8 mRNA expression and IL-8 release. CSE exerted its effects in a dose dependent manner. The increased miR-21 and IL-8 mRNA expression due to CSE exposure were reduced by carbocysteine and apigenin thus supporting the concept that oxidative stress sustained miR-21 expression and its related effects.In conclusions, data herein provided identified for the first time new mechanisms supporting the crucial role of the epithelium and of smoke induced oxidative stress in the amplification inflammatory responses within the airways.FootnotesCite this article as: European Respiratory Journal 2018 52: Suppl. 62, PA2171.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).