RT Journal Article
SR Electronic
T1 Evaluation of the airway microbiome in nontuberculous mycobacteria disease
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1800810
DO 10.1183/13993003.00810-2018
VO 52
IS 4
A1 Sulaiman, Imran
A1 Wu, Benjamin G.
A1 Li, Yonghua
A1 Scott, Adrienne S.
A1 Malecha, Patrick
A1 Scaglione, Benjamin
A1 Wang, Jing
A1 Basavaraj, Ashwin
A1 Chung, Samuel
A1 Bantis, Katrina
A1 Carpenito, Joseph
A1 Clemente, Jose C.
A1 Shen, Nan
A1 Bessich, Jamie
A1 Rafeq, Samaan
A1 Michaud, Gaetene
A1 Donington, Jessica
A1 Naidoo, Charissa
A1 Theron, Grant
A1 Schattner, Gail
A1 Garofano, Suzette
A1 Condos, Rany
A1 Kamelhar, David
A1 Addrizzo-Harris, Doreen
A1 Segal, Leopoldo N.
YR 2018
UL http://erj.ersjournals.com/content/52/4/1800810.abstract
AB Aspiration is associated with nontuberculous mycobacterial (NTM) pulmonary disease and airway dysbiosis is associated with increased inflammation. We examined whether NTM disease was associated with a distinct airway microbiota and immune profile.297 oral wash and induced sputum samples were collected from 106 participants with respiratory symptoms and imaging abnormalities compatible with NTM. Lower airway samples were obtained in 20 participants undergoing bronchoscopy. 16S rRNA gene and nested mycobacteriome sequencing approaches characterised microbiota composition. In addition, inflammatory profiles of lower airway samples were examined.The prevalence of NTM+ cultures was 58%. Few changes were noted in microbiota characteristics or composition in oral wash and sputum samples among groups. Among NTM+ samples, 27% of the lower airway samples were enriched with Mycobacterium. A mycobacteriome approach identified Mycobacterium in a greater percentage of samples, including some nonpathogenic strains. In NTM+ lower airway samples, taxa identified as oral commensals were associated with increased inflammatory biomarkers.The 16S rRNA gene sequencing approach is not sensitive in identifying NTM among airway samples that are culture-positive. However, associations between lower airway inflammation and microbiota signatures suggest a potential role for these microbes in the inflammatory process in NTM disease.16S rRNA gene sequencing is not sensitive to detect Mycobacterium but identifies microbiota signatures associated with inflammation http://ow.ly/opXm30ldtQH