RT Journal Article SR Electronic T1 Hypoxia-induced PD-L1/PD-1 crosstalk impairs T-cell function in sleep apnoea JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP 1700833 DO 10.1183/13993003.00833-2017 VO 50 IS 4 A1 Carolina Cubillos-Zapata A1 Jose Avendaño-Ortiz A1 Enrique Hernandez-Jimenez A1 Victor Toledano A1 Jose Casas-Martin A1 Anibal Varela-Serrano A1 Marta Torres A1 Isaac Almendros A1 Raquel Casitas A1 Isabel Fernández-Navarro A1 Aldara Garcia-Sanchez A1 Luis A. Aguirre A1 Ramón Farre A1 Eduardo López-Collazo A1 Francisco García-Rio YR 2017 UL http://erj.ersjournals.com/content/50/4/1700833.abstract AB Obstructive sleep apnoea (OSA) is associated with higher cancer incidence, tumour aggressiveness and cancer mortality, as well as greater severity of infections, which have been attributed to an immune deregulation. We studied the expression of programmed cell death (PD)-1 receptor and its ligand (PD-L1) on immune cells from patients with OSA, and its consequences on immune-suppressing activity. We report that PD-L1 was overexpressed on monocytes and PD-1 was overexpressed on CD8+ T-cells in a severity-dependent manner. PD-L1 and PD-1 overexpression were induced in both the human in vitro and murine models of intermittent hypoxia, as well as by hypoxia-inducible factor-1α transfection. PD-L1/PD-1 crosstalk suppressed T-cell proliferation and activation of autologous T-lymphocytes and impaired the cytotoxic activity of CD8+ T-cells. In addition, monocytes from patients with OSA exhibited high levels of retinoic acid related orphan receptor, which might explain the differentiation of myeloid-derived suppressor cells. Intermittent hypoxia upregulated the PD-L1/PD-1 crosstalk in patients with OSA, resulting in a reduction in CD8+ T-cell activation and cytotoxicity, providing biological plausibility to the increased incidence and aggressiveness of cancer and the higher risk of infections described in these patients.PD-L1/PD-1 crosstalk is upregulated in obstructive sleep apnoea patients and immunomodulates T-cell response http://ow.ly/gBEx30dZ7dd