RT Journal Article
SR Electronic
T1 IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP ERJ-00969-2016
DO 10.1183/13993003.00969-2016
A1 Annika Bergauer
A1 Nina Sopel
A1 Bettina Kroß
A1 Tytti Vuorinen
A1 Paraskevi Xepapadaki
A1 Scott T. Weiss
A1 Ashley Blau
A1 Himanshu Sharma
A1 Cornelia Kraus
A1 Rebekka Springel
A1 Manfred Rauh
A1 Susanne Mittler
A1 Anna Graser
A1 Theodor Zimmermann
A1 Volker O. Melichar
A1 Alexander Kiefer
A1 Marek L. Kowalski
A1 Anna Sobanska
A1 Tuomas Jartti
A1 Heikki Lukkarinen
A1 Nikolaos G. Papadopoulos
A1 Susetta Finotto
YR 2016
UL http://erj.ersjournals.com/content/early/2016/11/11/13993003.00969-2016.abstract
AB We analysed the influence of rhinovirus (RV) in nasopharyngeal fluid (NPF) on type I and III interferon (IFN) responses (e.g. IFN-α and IFN-λ) and their signal transduction, at baseline and during disease exacerbation, in cohorts of pre-school children with and without asthma.At the time of recruitment into the Europe-wide study PreDicta, and during symptoms, NPF was collected and the local RV colonisation was analysed. Peripheral blood mononuclear cells (PBMCs) were challenged in vitro with RV or not. RNA was analysed by quantitative real-time PCR and gene arrays. Serum was analysed with ELISA for IFNs and C-reactive protein.We found that PBMCs from asthmatic children infected in vitro with the RV1b serotype upregulated MYD88, IRF1, STAT1 and STAT2 mRNA, whereas MYD88, IRF1, STAT1 and IRF9 were predominantly induced in control children. Moreover, during symptomatic visits because of disease exacerbation associated with RV detection in NPF, IFN-α production was found increased, while IFN-λ secretion was already induced by RV in asthmatic children at baseline.During asthma exacerbations associated with RV, asthmatic children can induce IFN-α secretion, indicating a hyperactive immune response to repeated respiratory virus infection.Asthma exacerbations associated with rhinovirus in children induce IFN-α and STAT1/STAT2 in PBMCs http://ow.ly/o14r303MW32