@article {UmpelevaPA2787, author = {Tatiana Umpeleva and Kseniya Belousova and Leonid Lavrenchuk and Nataliya Eremeeva and Marionella Kravchenko and Diana Vachrusheva}, title = {Molecular-genetic and microbiological study of lung resection tissues from tuberculosis patients in Ural region, Russia}, volume = {48}, number = {suppl 60}, elocation-id = {PA2787}, year = {2016}, doi = {10.1183/13993003.congress-2016.PA2787}, publisher = {European Respiratory Society}, abstract = {Background: The spread of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of M. tuberculosis (MBT) has led surgery as a possible adjunctive treatment. Resectedlung tissue is a good material to diagnose verification and characteristic of infection agent.Objectives: Evaluate effectiveness of microbiological and molecular-genetic methods for diagnostic M. tuberculosis and its biological characteristic in surgical material.Material and methods: Total of 386 lung tissues samples were obtained from patients undergoing pulmonary resection at Ural Research Institute for Phthisiopulmonology. After resection tissue were dissected for microbiology and molecular-genetic investigation.Results: Of 386 specimens 28,2\% were microscopy positive, 12,7\% had positive culture. DNA MBT were detected in 95,6\% ceases and in 88,1\% it was enough for drug mutation detection. A total of 251 (65\%) isolates were genotyped: 82\% belong to Beijing lineage, 15\% {\textendash} to non-Beijing. 3\% of specimens had mix of genotype. Beijing isolates in 65,5\% ceases were MDR, 6,3\% - XDR while only 7,9\% non-Beijing isolates were MDR and no XDR ceases. In 15,1\% Beijing and 55,3\% non-Beijing isolates drug resistant mutation were not detected.Conclusion: The uses of molecular-genetic methods are more reliable for diagnostic MBT and its characteristic in surgical material. Beijing lineage is main cause of MDR and XDR tuberculosis in Ural region.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/48/suppl_60/PA2787}, eprint = {https://erj.ersjournals.com/content}, journal = {European Respiratory Journal} }