RT Journal Article
SR Electronic
T1 Effect of high, medium, and low molecular weight hyaluronan on inflammation and oxidative stress in an in vitro model of human nasal epithelial cells
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PA5064
DO 10.1183/13993003.congress-2016.PA5064
VO 48
IS suppl 60
A1 Albano, Giusy D.
A1 Bonanno, Anna
A1 Cavalieri, Luca
A1 Ingrassia, Eleonora
A1 Di Sano, Caterina
A1 Siena, Liboria
A1 Riccobono, Loredana
A1 Gagliardo, Rosalia
A1 Profita, Mirella
YR 2016
UL http://erj.ersjournals.com/content/48/suppl_60/PA5064.abstract
AB We investigated the pro or anti-inflammatory Hyaluronan (HA) activity at different molecular weights (MW) in an in vitro model of nasal inflammation IL-17A mediated. We evaluated the cytoplasmic ERK1/2 and IkBα phosphorylation,nuclear NF-kB signal pathway activation, ROS production, IL-8 and NOX-4 protein and mRNA levels, in nasal epithelial cells RPMI 2650 stimulated with recombinant human (rhIL-17A). The cells were treated with rhIL-17A in the presence or absence of HMW-HA (High-MW-HA; ∼1600 kDa), MMW-HA (Medium-MW-HA;∼900 kDa), LMW-HA (Low-MW-HA;500 kDa), and U0126 (specific inhibitor of MEK1and MEK2 MAP kinase kinase; MAPKK). We showed that rhIL-17A increased the ERK1/2, IkBα phosphorylation, NF-kB signal pathway activation, ROS production, IL-8 and NOX-4 proteins and mRNA levels. The addiction of HMW-HA or U0126, rather than MMW-HA or LMW-HA, showed a statistical significant down-regulatory effect on ERK1/2and IkBα phosphorylation, NF-kB signal pathway activation,ROS production, IL-8 and NOX-4 proteins in nasal epithelial cells stimulated with rhIL-17A. IL-17A might generate oxidative stress and inflammation via the activation ERK1/2/NFkB pathway in nasal epithelial cells. The HMW-HA might represent a coadjuvant of the classic anti-inflammatory/anti-oxidative treatment of nasal epithelial cells during IL-17A nasal inflammation.